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150ng 30μL 16s 18s RRNA ITS Region Full Sequencing Cellular Molecular Biology

150ng 30μL 16s 18s RRNA ITS Region Full Sequencing Cellular Molecular Biology
150ng 30μL 16s 18s RRNA ITS Region Full Sequencing Cellular Molecular Biology

Large Image :  150ng 30μL 16s 18s RRNA ITS Region Full Sequencing Cellular Molecular Biology

Product Details:

Brand Name: CELLFREE
Model Number: CF-CM-009

Payment & Shipping Terms:

Packaging Details: vial
Delivery Time: 15 days
Detailed Product Description
Sample Type: Genomic DNA Amount: ≥ 150 Ng
CONCENTRATION: ≥5 Ng/μL VOLUME: ≥ 30 μl
PURITY: OD260/280=1.8-2.0 Turnaround Time: 15 Working Days
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30μL Cellular Molecular Biology

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150 ng Cellular Molecular Biology

Identification of the microorganisms and phylogenetic relationship determination

– analysis of the sequences coding 16S rRNA (bacteria) and 18S rRNA (fungi).

 

Highly conserved fragments are intercalated within the genes coding 16S RNA (bacteria) and 18S rRNA (fungi). The sequences of these polymorphic regions are unique for each pathogen, genus, or species, so analysis allows identification and determination of phylogenetic affinity. Such studies are essential for strain identification in environmental specimens (e.g., in an activated sludge).

 

16S/18S/26S rRNA and ITS region full sequencing is a service that analyzes sequences by amplifying ribosomal RNA genes of bacteria and fungi (filamentous fungus and yeast) and confirming homology of target microorganisms using an rRNA database (NCBI). CELLFREE controls and performs all processes from gDNA extraction of bacteria and fungi (filamentous fungus and yeast) to PCR amplification, purification, sequencing, and BI report.

 

Sample requirements

 

SAMPLE TYPE REMARKS AMOUNT FRAGMENT SIZE CONCENTRATION VOLUME PURITY
Genomic DNA - ≥ 150 ng - ≥5 ng/μL ≥ 30 μl OD260/280=1.8-2.0

 

Service

 

Division

Sample type

Genes to analyze

Additional analysis

Animal Barcoding Animal, insect, bird, fish, etc. COI 12S/16S rRNA, ITS
Plant Barcoding plant matK, rbcL rpoB, rpoC1, atpF-atpH, psbK-psbI. psbA-trnH

 

Turnaround Time

 

  • Within 15 working days from verification of sample quality (without data analysis ≤100 samples)

  • Additional 5 working days for data analysis

150ng 30μL 16s 18s RRNA ITS Region Full Sequencing Cellular Molecular Biology 0 

Contact Details
CELLFREE SCIENCE CO., LTD.

Contact Person: Pan Lin

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