Payment & Shipping Terms:
|Sample Type:||Genomic DNA||Amount:||≥ 150 Ng|
|CONCENTRATION:||≥5 Ng/μL||VOLUME:||≥ 30 μl|
|PURITY:||OD260/280=1.8-2.0||Turnaround Time:||15 Working Days|
30μL Cellular Molecular Biology,
150 ng Cellular Molecular Biology
Identification of the microorganisms and phylogenetic relationship determination
– analysis of the sequences coding 16S rRNA (bacteria) and 18S rRNA (fungi).
Highly conserved fragments are intercalated within the genes coding 16S RNA (bacteria) and 18S rRNA (fungi). The sequences of these polymorphic regions are unique for each pathogen, genus, or species, so analysis allows identification and determination of phylogenetic affinity. Such studies are essential for strain identification in environmental specimens (e.g., in an activated sludge).
16S/18S/26S rRNA and ITS region full sequencing is a service that analyzes sequences by amplifying ribosomal RNA genes of bacteria and fungi (filamentous fungus and yeast) and confirming homology of target microorganisms using an rRNA database (NCBI). CELLFREE controls and performs all processes from gDNA extraction of bacteria and fungi (filamentous fungus and yeast) to PCR amplification, purification, sequencing, and BI report.
|SAMPLE TYPE||REMARKS||AMOUNT||FRAGMENT SIZE||CONCENTRATION||VOLUME||PURITY|
|Genomic DNA||-||≥ 150 ng||-||≥5 ng/μL||≥ 30 μl||OD260/280=1.8-2.0|
Genes to analyze
|Animal Barcoding||Animal, insect, bird, fish, etc.||COI||12S/16S rRNA, ITS|
|Plant Barcoding||plant||matK, rbcL||rpoB, rpoC1, atpF-atpH, psbK-psbI. psbA-trnH|
Within 15 working days from verification of sample quality (without data analysis ≤100 samples)
Additional 5 working days for data analysis