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1 μg RNA Sequencing Nucleolus RNA Synthesis

Large Image : 1 μg RNA Sequencing Nucleolus RNA Synthesis

Product Details:

Brand Name:	CELLFREE
Model Number:	CF-RN-002

Payment & Shipping Terms:

Packaging Details:	vial
Delivery Time:	20~35 days

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Detailed Product Description

SAMPLE TYPE:	Total RNA	AMOUNT:	≥ 1 μg
VOLUME:	≥ 20 μl	CONCENTRATION:	≥ 20 Ng/μl
PURITY:	No Degradation, No Contamination	Recommended Sequencing Depth:	≥ 12Gb Data
High Light:	RNA Sequencing Nucleolus RNA Synthesis , RNA Sequencing Nucleolus Synthesis , 1 μg Nucleolus RNA Synthesis

RNA sequencing (RNA-Seq) is revolutionizing the study of the transcriptome. A highly sensitive and accurate tool for measuring expression across the transcriptome, it is providing researchers with visibility into previously undetected changes occurring in disease states, in response to therapeutics, under different environmental conditions, and across a broad range of other study designs.

RNA-Seq allows researchers to detect both known and novel features in a single assay, enabling the detection of transcript isoforms, gene fusions, single nucleotide variants, and other features without the limitation of prior knowledge.

Applications

Discover novel transcripts, identify intron/exon boundaries or discover alternative splicing isoforms
Identify specific strand information, genefusions or mutations/SNPs
Observe alterations in gene expression or mutation cancer
Determine host/pathogen interactions
Characterize bacterial pathogenesis, antimicrobial resistance or persistence
Identify viral quasi-species or latency activation

For your project, firstly we will give a detailed QC report of data from raw fastq file to aligned BAM file. Our QC analysis will tell you whether your data is suitable for some specific analysis such as gene fusion or splicing and whether your sequencing depth is enough for expression analysis.

For the result of your data, we will provide the high quality visualization figure of your data. The data visualization will include Heatmap, Pahway and Network. We may also provide the circos plot of the whole genome if applicable.

Sample requirements

LIBRARY TYPE	SAMPLE TYPE	AMOUNT	VOLUME	CONCENTRATION	RNA INTEGRITY NUMBER	PURITY
Eukaryotic RNA-Seq	Total RNA (Animal)	≥ 1 μg	≥ 20 μl	≥ 20 ng/μl	≥ 6.8, smooth base line	No degradation, no contamination
Eukaryotic RNA-Seq	Total RNA (Plant and Fungus)	≥ 1 μg	≥ 20 μl	≥ 20 ng/μl	≥ 6.3, smooth base line	No degradation, no contamination
Prokaryotic RNA-Seq	Total RNA	≥ 3 μg	≥ 20 μl	≥ 20 ng/μl	≥ 6.0, smooth base line	No degradation, no contamination
Low input RNA	Total RNA	≥ 200ng	≥ 20 μl	≥ 20 ng/μl	Animal: ≥ 6.8, Plant & Fungus: ≥ 6.3, smooth base line	No degradation, no contamination
Amplified cDNA	Double-stranded cDNA	≥ 200ng	-	-	Peak range: 400 bp - 9000 bp , Main peak: 1200 bp - 2500 bp	No degradation, no contamination