Product Details:
Payment & Shipping Terms:
|
| Starting Materials-1: | Antibody Sequence | Starting Material-2: | Hybridoma Cells |
|---|---|---|---|
| Product: | 1-3 Mg Antibody | QC: | Sequencing Report |
| Purity: | >90% | Time: | 3~4 Weeks |
| High Light: | 3 mg High Throughput Antibody Production,3 mg Recombinant Production |
||
Introduction
Recombinant antibodies are monoclonal antibodies which are generated in vitro using synthetic genes, have the advantages in specificity and low immunogenicity compared to traditional antibodies. They have been widely used in the research and application in the field of diagnosis and treatment therapies for various diseases.
Recombinant Antibody Expression
Combining our rich experience in both molecular biology and cell system engineering, CELLFREE has established as a world-leading expert in recombinant antibody expression. We have been providing high quality recombinant antibodies to support a variety of applications in drug discovery research, including high throughput screening (HTS), in vitro activity and functional assays, in vivo animal studies.
We use the proprietary transient mammalian expression technology to modify cellular pathways to achieve optimal antibody yield (up to 3 g/L). We also optimized expression protocol and growth media to maximize the cells longevity, for guaranteed gram-level recombinant antibody expression projects.
In addition to mammalian expression, we also offer E. coli and insect expression platforms that is ideal for high-quality recombinant antibody fragments (scFv, VHH, VHand Fab.) With expertise in both molecular biology and cell engineering, CELLFREE has been a reliable expert, assisting scientists in over 86 countries around the world for their recombinant antibody expression projects.
Service Content
|
Procedure |
Description |
Deliverables |
Timeline |
|---|---|---|---|
|
Customers provide |
Customers can provide any of the following three options:
|
Sequencing report (if sequencing) |
|
|
Subcloning and transfection |
Antibody genes are constructed into mammalian expression vector, and then transfected into CHO / HEK293 cells. |
1 μg constructed vectors with antibody gene |
1 week |
|
Transient expression & Purification |
After transient expression, we can purify 1-3 mg recombinant antibody with purity more than 90% using Protein A/G column. |
|
3 weeks |
|
QC |
ELISA and western blot is performed for detection. |
Western blot and ELISA report |
1 week |
