4 μg MiRNA Vector Construction Lyophilized Plasmid RNAi Vector Construction

Introduction

MicroRNAs (miRNAs) are a class of noncoding RNAs that regulate gene expression by single-stranded RNAs of 18 to 25 nucleotides in length. Hundreds of miRNAs have been found in animals and plants, some of which play important roles in development or differentiation. Increasing attention has thus been paid to their biogenesis and regulation mechanisms and the identification of target genes.

CELLFREE offer you an alternate way to clone your sequences of interest with our vector-based miRNA technology. In addition to our existing siRNA construction and the entire spectrum of siRNA vectors that we offer, we have launched a miRNA construction service in the vector of your choice. We can assist you with the construction of the gene sequence that you have chosen and provided us, that can be then cloned into any of our miRNA cloning vectors. The resulting miRNA expressing plasmid is purified and ready to transfect and can be used for a variety of applications such as quantitation using Northern blotting, dot blotting, RNAse protection assay, primer extension analysis, Invader assay and quantitative PCR.

Delivery Form

• Final product is confirmed by stringent sequencing. 4 μg of lyophilized plasmid DNA containing the desired miRNA construct
• Detailed sequencing data documenting the DNA sequence of each clone

Two Methods