Payment & Shipping Terms:
|Strains:||Pichia Pastoris, Saccharomyces Cerevisiae And So On||Expression Vector Construction:||2~5 µg Freeze Dried Plasmid|
|Transforming:||6 Colonies For Expression||Optimization Of Expression:||Approved By SDS-PAGE|
|Amplified Cultivation:||1-10 Mg Recombinant Proteins Offered||Yield:||2 Times Of Compared With E. Coli.|
5µg plasmid Protein Expression Purification,
Intracellular Expression Protein Expression Purification
Yeast protein expression system is a ideally suitable expression system for both secretion and intracellular expression as the yeast offer relatively easy genetic manipulation and rapid growth to high cell densities on inexpensive media.
Different strains of yeasts show different in their capabilities to secrete, process and modify proteins, meaning different types of yeast suit for different research and industrial applications. Common yeast protein expression systems are Pichia pastoris, Saccharomyces cerevisiae, Kluyveromyces lactis, Hansenula polymorpha, and Yarrowia lipolytica.
Expression vector construction (optional)
CELLFREE provides the service of directly cloning genes into commercial expression vectors such as methanol-induced pPICZα,pPIC9K or component pGAPZα, etc..
Delivery contents: 1 tube of freeze dried plasmid (2~5 µg/tube) and 1 tube of glycerol containing the plasmid TOP10
Delivery TAT: 10-20 business days
Linearized vector andelectricity transforming into pichiapastoris
You can also give us a constructed recombinant plasmid (please confirm the codon of the sequence is optimized). We transform them into the pichiapastoris. After the screening, we select at least 6 colonies for expression laboratory scale test.
Delivery contents: screening report of positive colonies
Delivery TAT: 5 business day
Optimization of expression conditions
During the process oflaboratory scale test,we will optimize and testthe culture conditions which are suitable for various strains, including temperature, culture medium, inducer type, induction time, induction quantity and other factors.It is tacitly approved that we use SDS-PAGE or Western blotto (optional, the customer provides a varified first antibody) to analyze the expression effect.
Delivery contents: A copy of laboratory scale test verification report
Delivery TAT: 5 business days
Amplified cultivation,expression and purification interest protein
To optimize and obtain the strains with appropriate expression conditions and use 1L or greater culture scale to induce recombinant yeast expression. After the induction, one step or multi-step purification is conducted.we use SDS-PAGE to verify the purification and Western blotto (optional, the customer provides a varified first antibody) to verify the accuracy.
Delivery contents: 1-10 mg recombinant proteins and purification report data
Delivery TAT: 10-15 working days
For protein swith tags, you can choose remove service (optional).
Protein post processing (optional)
For those proteins with further special requirements, Our experienced engineers can provide tailor-made post servicesfor your special needs.
Gram level protein production: Suitable for customized production of industrial enzymes.
High yield: Increase the protein yield by 2x compared with E. coli. A yield of 5-10g/L from yeast fermentation.
Stable production process: the target protein expression cassette is integrated into genome leading to stable protein expression process.