Home Products Protein Expression And Purification80% Purity 10mg Soluble Protein Recombinant Baculovirus Preparation Baculovirus Expression Vector System

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10mL Crude Supernatant Recombinant Protein Production

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80% Purity 10mg Soluble Protein Recombinant Baculovirus Preparation Baculovirus Expression Vector System

Large Image : 80% Purity 10mg Soluble Protein Recombinant Baculovirus Preparation Baculovirus Expression Vector System

Product Details:

Brand Name:	CELLFREE
Model Number:	CF-PR-005

Payment & Shipping Terms:

Packaging Details:	vial
Delivery Time:	1~2 weeks

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Detailed Product Description

Customer Offer:	Plasmids Or Sequeces Of Target Protein	We Offer:	Expression And Evaluation
Report:	Construction Report	Deliverables:	1-10 Mg Soluble Protein
Purity:	> 80%	Electronic Data:	Western Blot Report And QC Report
High Light:	10mg Soluble Protein Expression Vector System , 80% Purity Baculovirus Expression Vector System

Introduction

The baculovirus expression vector system (BEVS) is one of the four major expression systems of genetic engineering. BEVS has several advantages of using the baculovirus-insect cell expression system, such as improved solubility and comprehensive post-translational modifications.

With our experienced scientific team, CELLFREE has developed our own baculovirus-insect cell expression technology platform. The production scale of this platform is up to 500 L. Combined with our advanced protein purification facilities, we can deliver recombinant protein products with purity 95% and above. Protein expression levels and success rates can be significantly improved by us.

Service Content

Provide	Services	Deliverables
Plasmids or sequeces of target protein	Gene synthesis Subcloning Construction of baculovirus plasmid Transfection Screening Expression and evaluation Purification and detection	Construction report 5 ml virus with more than 7 pfu/ml Expression data Western blot report QC report 1-10 mg soluble protein with a purity > 80%

Package details

Steps	Specifications	Timeline^#	Deliverables
Gene synthesis and/or subcloning	Gene synthesis and codon optimization using our proprietary platforms (optional) Subcloning into 1 or more expression vectors	2 weeks	Construct(s) report
Virus generation	Generation of recombinant Bacmid DNA Transfection of insect cell with recombinant Bacmid DNA Generation of P1 stock (low titer), P2 stock (high titer), and determination of virus titer by quantitative-PCR Protein expression verification using anti-His or anti-GST antibody	2 weeks	5 ml, >10⁸ pfu/ml virus stock
Expression	Infection of insect cells with P2 stock SDS-PAGE gel and Western blot	1 weeks	QC data

For customers who wish to establish the best possible protocol for their project and then scale up, CELLFREE recommends using the sophisticated platform to explore different conditions for the expression of target proteins.
80% Purity 10mg Soluble Protein Recombinant Baculovirus Preparation Baculovirus Expression Vector System 0

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Contact Person: Pan Lin