85% Purity 0.5 Mg/Ml Fluorescent Antibody 1mg Minimum Modifications Conjugation Antibody Discovery

Introduction

Our custom fluorescent antibody and protein labeling service combines years of conjugation expertise with a wide selection of labels, including fluorescent dyes, phycobiliproteins, and enzymes, along with a choice of labeling chemistries. We provide conjugation services for both research and commercial use.

Benefits of iFluor labeling

• Greater photo-stability: The energy used to excite a fluorophore and drive subsequent light emission, can also destroy fluorescence. Known as "photobleaching," prolonged exposure to light chemically destroys the dye through non-reversible cleavage of covalent bonds. This can be problematic for repeated imaging of samples, or during extended periods of sampling such as those involved in time-lapse imaging. FITC technology allows for durable fluorescence due to improved resistance to photobleaching compared to alternative dyes of comparable wavelengths.

• Stronger absorption and fluorescence: Fluorophore performance depends upon the ability to absorb and emit the greatest amount of energy within its intended wavelength. iFluor commonly outperforms other fluorophores emission strength, making for extremely sensitive and robust detection of your labeled antibody.

• Resilient signals in varying pH: Harsh environments such as lysosomes can degrade typical fluorophores, negatively impacting their performance. iFluor pH-insensitive dyes offer optimal signal detection from pH 3 to 11.

*For best results, we recommend starting with at least 1 mg of material, but can often accommodate smaller conjugations, depending on the protein.

In most cases, we can ship product within 15 business days of receiving your antibody and order. Some projects will require additional time.

More services

Antibody customization doesn't stop after antibody generation. Whether using your custom antibody in immunohistochemistry (IHC), or fluorescent activated cell sorting (FACS), conjugating your antibody allows for simplified detection. Labeling of your antibody with horseradish peroxidase (HRP) provides for sensitive enzymatic detection in IHC. Biotin labeling allows for the flexibility of using a variety of labeled avidin. Finally, labeling your antibody with fluorophores offers a means of direct detection, eliminating the need to juggle multiple secondary antibodies when visualizing or flow sorting with multiple markers. Include our conjugation and labeling services with your custom antibody production for streamlined processing, and even cost savings.