AAALAC Mammalian Cells Mammalian Gene Editing Service Crispr Genome Editing

Introduction

CRISPR/Cas9 technology is a powerful tool for creating gene knock-ins and knock-outs.
 

CRISPR/Cas technology has quickly become one of the easiest and most effective genome editing systems, and when combined with λ Red recombineering has several advantages over the most common editing strategies:

1. Wide range of applications
   CRISPR-Cas9 genome editing technology has no restrictions among genomic sequences, cell types, or species.
2. Simple and convenient to construct
   CRISPR-Cas9 requires only a short sequence, approximately 20bps in length, of the sgRNA sequence to complete target recognition.
3. Efficient turnaround time
   Genetic stability of homozygous strains can be constructed within an efficient timeframe.
4. Multiple site knock out
   Multiple sites can be targeted at the same time to successfully accomplish multi-site knockout.

Advantages of our service

1. One-stop service
2. Proprietary technology platform
3. sgRNA library synthesis
4. High-throughput function screening platform

Mammalian Gene Editing Service list

• sgRNA Endogenous Activity Detection

1. A variety of sgRNA endogenous activity assays guarantee the efficiency of the generated gene knock-out.
2. The methods of detection include: SSA activity detection, in vitro cleavage activity detection, and endogenous activity detection.

• Lentiviral Package

Lentiviral package services offer: transfection of mammalian cells, facilitation of stable Cas9 protein-expressing cell lines, and improving gene knock-out efficiency.

• Small Animal Gene Knock-out

With our experienced team of R&D engineers, CELLFREE is capable of providing gene knockout services for zebrafish, mouse, and rat genomes.

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