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Antigen Sequence Hybridoma Technology Bulk Antibody Discovery

Antigen Sequence Hybridoma Technology Bulk Antibody Discovery
Antigen Sequence Hybridoma Technology Bulk Antibody Discovery

Large Image :  Antigen Sequence Hybridoma Technology Bulk Antibody Discovery

Product Details:

Brand Name: CELLFREE
Model Number: CF-AB-008

Payment & Shipping Terms:

Packaging Details: vial
Delivery Time: 8~14 weeks
Detailed Product Description
Product: Bulk Antibody Quantity Level: MG To G
Method: Hybridoma Technology QC: Test Report
Other Information: Expression Report Starting Information: Antigen Sequence
High Light:

Hybridoma Technology Bulk Antibody Discovery

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Antigen Sequence Bulk Antibody Discovery

Introduction of Large-scale Antibody Manufacturing Services

 

Manufacturing antibodies in large quantities is required for many applications, including pre-clinical trials of antibody drugs. CELLFREE's large-scale antibody manufacturing services offer scale-up production of monoclonal and polyclonal antibodies from milligram to gram levels suitable for any application.

 

Service content

 

Phase

Process

I : Receipt of client cell line

We will receive your cell line as a frozen ampoule of growing culture

 

Initial growth in serum supplemented medium followed by mycoplasma analysis using PCR

II : Pilot 1 L production

Adaptation of cell line onto protein-free medium

 

Scale-up in roller bottle culture, typical yield of 20-40mg of purified antibody

III : Downstream processing

Harvesting of cell culture supernatant

 

Concentration by tangential cross flow filtration

Protein A/G purification

Final buffer formulation

IV : QC assessment of antibody purity

Analysis of purity by SDS-PAGE, WB, ELISA and aggregation assessment by SEC

V : Antibody scale-up

Milligramme to Gramme quantities

 

Scale up performed using bioreactor system

Purification using Protein A/G affinity chromatography

 

Final Deliverables

  • Purified monoclonal antibody, protein, or concentrated supernatant to requested scale (up to 250L)

  • Working Cell Bank for Scale-up cell line

  • Project report with ELISA, SDS-PAGE, Western Blot, and SEC performance data

 

Features & Benefits

  • Rat monoclonal antibody using ascites production

  • A nude mice facility for ascities production can be used as an alternative method to get monoclonal antibodies which cannot be produced in vitro system

  • Size ranging from milligrams to grams

  • Serum-free to lower contamination of bovine immunoglobulin

  • A lower endotoxin level making possibility of antibodies application in vivo

  • The very competitive price in the industry

  • Full privacy on your project

Antigen Sequence Hybridoma Technology Bulk Antibody Discovery 0 

Contact Details
CELLFREE SCIENCE CO., LTD.

Contact Person: Pan Lin

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