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Codon Optimization DNA Sequences Protein Expression And Purification

Codon Optimization DNA Sequences Protein Expression And Purification
Codon Optimization DNA Sequences Protein Expression And Purification

Large Image :  Codon Optimization DNA Sequences Protein Expression And Purification

Product Details:

Brand Name: CELLFREE
Model Number: CF-PR-012

Payment & Shipping Terms:

Packaging Details: vial
Delivery Time: 2~5 working days
Detailed Product Description
Customer Off-1er: Target Sequence Customer Offer-2: Desired Number Of Constructs
Report: DNA Sequences, Protein Expression Screening Analysis Constructs: Recommended
Optional-1: Downstream Protein Expression Optional-2: Stable Cell Line Development
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Codon Optimization Protein Expression Purification


DNA Sequences Protein Expression Purification

Codon optimization is a novel technique to improve protein expression level in living organism by increasing translational efficiency of target gene. CELLFREE provides state-of-the-art algorithms to optimize gene sequences using in-house pre-computed software from a predicted group of highly expressed genes from thousands of samples.


The demand for recombinant proteins has increased significantly during the past decades, this has in turn increased the need for producing sufficient amounts of high-quality protein samples for various applications. CELLFREE codon optimization and verification services are designed to facilitate the identification of optimal genetic combinations for enhancing recombinant protein expression. 




  • Express a novel protein target
  • Increase production level of a poorly expressed protein
  • Improve antibody production for downstream process development
  • Enhance protein expression level to reduce production cost


Key Features


  • Significant improvement in expression: Combinatorial approach for success
  • Comprehensive coverage: Optimization for bacteria, yeast, mammalian and plant system
  • Guaranteed result: Full report delivered for each combination tested


Service Specifications


Customer Provides Service Specifications Deliverables
  • • Target sequence (or accession number) to be optimized for Expression system to be optimized for: E. coli, Pichia pastoris, mammalian cells (CHO/HEK293), insect cells (sf9), green algae
  • • Desired number of constructs/sequences to be evaluated
  • 1. Construct optimization*: Sequence Optimization; Gene Synthesis
  • 2. Subcloning to expression vector(s)
  • 3. Experimental verification: Protein expression screening; SDS PAGE analysis; Test affinity purification when applicable
  • • Full report including optimization of DNA sequences, protein expression screening analysis and our recommended constructs
  • • >2µg DNA of expression optimized construct with sequence file
  • • Professional consultation on data analysis
  • • (Optional) Downstream protein expression/purification, scale up production, stable cell line development services are also available upon request




Contact Details

Contact Person: Pan Lin

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