CRISPR Oligo Pool DNA UV Oligo Pool Oligo Pool Synthesis

DNA oligo pool is a mixture of large numbers of different oligos in fmole to nmole range. CELLFREE can synthesize DNA oligo pools according to customer specifications.

Many aspects of biological research such as genetic library construction, target enrichment/capture in Next Generation Sequencing (NGS), require a large amount of primers. Conventional approaches to synthesizing hundreds of thousands of primers at a time are ineffective and time consuming. CELLFREE offers the revolutionary approach towards large-scale gene synthesis with fast turnaround times and overall cost. This is accomplished by leveraging precise electrochemical technology, tens of thousands of oligos can be in parallel synthesis on a single chip within one synthetic circle. The individually synthesized primer strands are subsequently eluted from the chip base to allow the mixture of a variety of oligos. We focus on commercialization efforts to manufacture DNA oligo pools to satisfy customers’ research needs.

Service Features

1. Oligo length range from 5-125 bases, pool size up to 10^3-4

2. Chip synthesized or plate synthesized

3. Range from fmole to nmole depending on synthesis method

4. Amount of each oligo can be equalized

5. Low error rate

 Service Specifications

 *If you need oligos of 171-200 bases in length, please contact us for pricing and availability. Pricing is determined by the longest oligo length in the pool. We recommend keeping the lengths of the oligos in your pool within 10%.

Applications for Oligo Pool Service

Oligo pools have a wide array of uses in various research applications. Click below to learn more about how GenScript’s oligo pool and related services can meet your application needs.

1. Genome Editing

2. Protein & Antibody Engineering

3. NGS Target Enrichment

4. Synthetic Biology

QC contents

1. Performance of all electrodes are verified and logged for each oligo pool
2. Post-synthesis visual verification of each synthesized DNA for each electrode by microarray scanner.
3. Purity and yield of oligo pool is tested by UV full spectrum analysis
4. PCR amplification (Optional)
5. Cloning and sequencing to check the sequence coverage and accuracy (Optional)
6. Guaranteed final yield of synthesized oligos in each pool