Payment & Shipping Terms:
|Information 1:||Gene Information||Information 2:||Mutation Site Sequence Information|
|Raw Material:||Wild Type Plasmi (dplasmid ≥ 2 μg)||Acceptable Map:||Sequencing Map, Vector Map|
|Report 1:||Sequencing Report||Report 2:||Single Experiment Report|
DNA Sequence Analysis Site Directed Mutagenesis,
Wild Type Plasmi Site Directed Mutagenesis
Site-directed mutagenesis is an invaluable tool to modify genes and study the structural and functional properties of a protein, based on the structure, function, catalytic mechanism, and catalytic residues of enzymes. Site-directed mutagenesis includes single and combinational mutations. It is usually analyzed by bioinformaticmethods. Single site-directed mutagenesis and multiple mutations have been used to expedite and simplify methods for mutagenes.
Site-directed mutagenesis will be carried out on the selected sequence to make specified base changes. The strategy may involve a PCR mutagenesis approach, or an oligonucleotide-directed approach, depending on the needs and specifics of the project.
All mutagenesis clones are screened by DNA sequence analysis to ensure that the desired base changes have been made, and to ensure sequence integrity of the remainder of the clone.
You will receive a mini-scale plasmid preparation, and a stab culture of the strain carrying the plasmid, along with a report documenting restriction analysis and DNA sequence analysis.