Exchange Chromatography 0.1EU/Mg Bacterial Expression LPS Removal Low Lipopolysaccharides Endotoxin Removal

Introduction

The E.coli as the host cell is typically used for the prokaryotic expression system for several reasons. The cells have advantages such as rapid proliferation, high expression, easy purification, good stability, strong anti-pollution ability and cost effectiveness. This is why the bacterial expression system is not only the most mature and reliable, but also the most commonly used, economical and effective option for protein expression. At the stage of actively growing, E.coli cell sheds small amounts of endotoxins into its surroundings, however, large amounts can be released while it dies. Endotoxins can stimulate the mammalian immune system and activate complement by the alternative (properdin) pathway, resulting in a strong immune response such as shock, organ failure, tissue injury, disseminated intravascular coagulation, and even death. Endotoxins can also strongly influence transfection of DNA into primary cells, and lead to reduced transfection efficiencies.

CELLFREE has the expertise in endotoxin removal and detection service. Our advanced equipments, high skilled teams and professional technologies are available to offer you with a reliable and accurate endotoxin removal and detection service to meet your specific requirments.

Service Process

Endotoxins themselves are difficult to remove or inactivate because they are extremely heat and pH stable. They can be removed by some other ways.

1. Due to negatively charged of the endotoxins (above a pH of 2.0), a positively charged membrane surface can remove endotoxin.

2. The hydrophobic interaction of the lipid A component of the endotoxins and hydrophobic membrane, hydrophobic filter devices can be used for removing endotoxin from buffer/ionic solutions.

3. Strong anion exchange chromatography has proven to be particularly effective at removing endotoxins from feedstreams becouse of the charged nature of endotoxins.

​Endotoxin Removal

For each endotoxin removal project, we evaluate the target protein, estimate efficiency of removal and loss rate of target protein, and customize the most suitable protocol for each project. Then we perform the customized protocol to remove endotoxins from target proteins, followed by LAL assay.

Endotoxin Detection

The detection of endotoxin performs before and after endotoxin removal. The primary methods can be divided into qualitative (LAL gel-clot assay) or quantitative (LAL turbidimetric assay and LAL chromogenic assay).

Customized Protein Service Advantages

1. Ideal for challenging proteins and other difficult projects
2. Flexible and affordable service tailored to meet your demands
3. Fast turnaround - as little as 4 weeks to get your purified proteins
4. One-stop service platform from gene to protein
5. Low-endotoxin level and secreted proteins