Express Cloning Molecular PCR Cloning Lyophilized Plasmid DNA Biobasic Gene Synthesis

Express Cloning Molecular Subcloning Service Customized Constructs PCR Cloning

To get expression-ready constructs containing a DNA sequence you've designed or that is known from GenBank or other sources, it's usually more efficient to start with 100% sequence verified gene synthesis rather than wasting time trying to clone template DNA that may be of poor quality, truncated, chemically modified or contain contaminats due to long storage time or prior handling. Cloning can be tedious and time-consuming depending on your project needs. Let CELLFREE generate your desired constructs so you can focus on the steps that are critical to your research. Our cloning services are designed to quickly deliver customized constructs with 100% accuracy. Your gene of interest can be obtained by PCR or restriction digestion and cloned into the vector of your choice, or you can outsource DNA cloning projects from templates you already have.

Features & Benefits

1. No more bench work to prepare the construct before you can start your experiments.

2. No more work to order multiple kits and services from multiple vendors to gather all the reagents you need.

3. No more risk of getting the wrong construct, as we ships only 100% sequence-verified constructs with sequence chromatograms.

What we need

1. Protein sequence or ORF DNA sequence.
2. Intended Latin name of host expression system.
3. Restriction sites at both ends.
4. Restriction sites that you want to avoid in the optimized sequence.
5. Restriction sites that you want to keep in the original sequence.

Service Content

*Turnaround times listed are estimates based on non-complex orders, some complex sequences may require additional time for synthesis. For a more accurate estimate, please request a quote.
*Orders are deliverable in either single tube or 96-well PCR plate formats at no additional charge.