Product Details:
Payment & Shipping Terms:
|
| Application: | Research, Industrial Production | Deliverables For Researchent: | Single Cell Clone, Cell Pool |
|---|---|---|---|
| Timeline: | 3 Months | Deliverables For Industry: | Expression Vector |
| Report: | Sequencing Report | ||
| High Light: | Cell pool Stable Knockdown Cell Line,Sequencing Report Stable Knockdown Cell Line,Expression Vector Stable Knockdown Cell Line |
||
Introduction
Stable cell lines are used in a variety of research applications such as recombinant protein and antibody production, assay development, gene editing, functional studies and more. CELLFREE offer comprehensive stable cell line development capabilities that either begin with DNA provided by our clients or that are powered by our fast, high efficiency gene synthesis service that seamlessly integrates all steps of stable cell line generation, delivering high quality stable clones of your choice. Boost your productivity and improve your results by leveraging CELLFREE's top quality stable cell line services.
Cell Line Construction for Research
We can insert the gene you are interested in into the genome of any cell and screen the stably integrated monoclonal cell line which can be applied in many research fields, such as signal transduction, drug target study, immunotherapy drug development, etc. We have successfully constructed cell lines in dozens of kinds of cells. All you need to do is to provide the gene sequence, and we will complete the screening of cell lines as required.
Service content
|
Content |
Deliverables |
Timeline |
|---|---|---|
| Cell Line Construction | Single cell clone | 3 months |
| Cell pool | 2 months |
Service Process
Cell Line Development for Industrial Production
The cell for stable cell line development is CHO cell. The CHO cells that are directionally domesticated for our self-developed highly-expressed proEM system show high productivity and cell density in the production of recombinant protein. It can also be domesticated on the basis of client’s special culture medium components. It has excellent growth and metabolism characteristics. Whether in shake flask, wave or bioreactor, its strong growth curve lays a solid foundation for high yield of protein.
Service content
|
Process |
Deliverables |
Timeline |
|
|---|---|---|---|
| Gene Synthesis |
Codon optimization Gene synthesis Vector construction Plasmid Max-Prep |
Expression vector Sequencing report
1 Cell Line tubes/plant, 1×107cells/ml) |
4 weeks |
| Cell Line Development |
Cell transfection Expression evaluation MTX/MSX amplification Single cell clone screening |
3 months | |
