Gene Sequence 1000 Mg/L Process Optimization Protein Expression And Purification

CELLFREE possesses a mature protein expression system and advanced protein expression technology. Our fermentation services include normal fermentation, high-density fermentation, and process optimization. We have a variety of fermentation tanks, bioreactors and protein purification systems, which can provide recombinant proteins from milligram level to gram level. The expression system and fermentation protocol can be customized according to customers’ specific needs.

Service Process

• Gene optimization
• Process optimization
• Fermentation
• Protein purification

Gene Optimization

Gene sequence is a key factor in the success of protein expression. CELLFRE can effectively optimize the genes (such as mRNA structure, rare codon, ribosomal binding site, etc.).

Optimization of Expression Conditions

We optimize protein expression conditions, including temperature, inducer, OD and etc., leading higher cell culture density and protein expression in subsequent fermentation.

Fermentation

Depending on the different requirements of customers, CELLFREE provides normal fermentation and high-density fermentation. We optimize the fermentation process, including temperature, pH, dissolved oxygen, feeding mode and so on. In addition, fed-batch strategy is the most effective method to achieve a high yield for high-density fermentation.

High-density Fermentation

The fed-batch strategy is an effective method to achieve high yield recombinant protein in high-density fermentation. The quality and productivity of cells can be maximized by adjusting the culture conditions, including temperature, PH value and the feed rate of culture and substrate.

Advantages

1. Professional technical team
2. Mature and stable protein expression system
3. Comprehensive clone and express strains
4. Guaranteed amount and purity of custom protein
5. Competitive price.