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Gene Sequence 1000 Mg/L Process Optimization Protein Expression And Purification

Gene Sequence 1000 Mg/L Process Optimization Protein Expression And Purification
Gene Sequence 1000 Mg/L Process Optimization Protein Expression And Purification

Large Image :  Gene Sequence 1000 Mg/L Process Optimization Protein Expression And Purification

Product Details:

Brand Name: CELLFREE
Model Number: CF-PR-005

Payment & Shipping Terms:

Packaging Details: vial
Delivery Time: 1 to 12 weeks
Detailed Product Description
Normal Fermentation: 5-50 Mg/L High-density Fermentation: 20-400 Mg/L
Process Optimization: 100-1000 Mg/L Report: Experimental Report&COA
Timeline: 1 To 12 Weeks Key Factor: Gene Sequence
High Light:

1000 Mg/L Protein Expression Purification

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Gene Sequence Protein Expression Purification

CELLFREE possesses a mature protein expression system and advanced protein expression technology. Our fermentation services include normal fermentation, high-density fermentation, and process optimization. We have a variety of fermentation tanks, bioreactors and protein purification systems, which can provide recombinant proteins from milligram level to gram level. The expression system and fermentation protocol can be customized according to customers’ specific needs.

 

Service Process

 

  • Gene optimization
  • Process optimization
  • Fermentation
  • Protein purification

 

Gene Optimization

 

Gene sequence is a key factor in the success of protein expression. CELLFRE can effectively optimize the genes (such as mRNA structure, rare codon, ribosomal binding site, etc.).

 

Optimization of Expression Conditions

 

We optimize protein expression conditions, including temperature, inducer, OD and etc., leading higher cell culture density and protein expression in subsequent fermentation.

 

Fermentation

 

Depending on the different requirements of customers, CELLFREE provides normal fermentation and high-density fermentation. We optimize the fermentation process, including temperature, pH, dissolved oxygen, feeding mode and so on. In addition, fed-batch strategy is the most effective method to achieve a high yield for high-density fermentation.

 

High-density Fermentation

 

The fed-batch strategy is an effective method to achieve high yield recombinant protein in high-density fermentation. The quality and productivity of cells can be maximized by adjusting the culture conditions, including temperature, PH value and the feed rate of culture and substrate.

 

Advantages

 

  1. Professional technical team
  2. Mature and stable protein expression system
  3. Comprehensive clone and express strains
  4. Guaranteed amount and purity of custom protein
  5. Competitive price.

Contact Details
CELLFREE SCIENCE CO., LTD.

Contact Person: Pan Lin

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