HPLC Report Nuclei Acid Probes RP-HPLC Oligonucleotide Probe Synthesis

Introduction

Nuclei acid probes are sequences of single stranded DNA or RNA fragments that have the potential to bind specifically to their complementary nucleic acid sequences through a process called hybridization. Nuclei acid probes are usually labeled with a detectable moiety such as radioisotopes, epitopes, fluorophores or biotin to facilitate the detection of their respective target molecules. Recently, nuclec acid probes are becoming a routinely used tool in various research areas. This increased prevalence is due to the increased availability of nuclei acid sequences in databases and advances in molecular biology, such as PCR and NGS. A few examples of these advances include: molecular diagnosis, chip sequencing, human genome engineering, tumor research, forensic identification, precision medicine, IVD, etc.

With our propriety synthesis platform and professional research team, CELLFREE is able to produce high quality nucleic acid probes with batch consistency for both routine and specialized applications. Additionally, we can provide a wide variety of modifications or labeling at the 5′ and 3′ ends or a designated position of the probe according to the specific need of our customers.

It ranges from unmodified oligonucleotides (helper probes) and standard fluorescently mono-labeled FISH probes to oligonucleotide-enzyme conjugates (CARD-FISH) or even dual-labeled probes for highly specific tasks (systematic investigations on hybridization parameters/method development). Due to reasonable prices today offered by companies specialised on biopolymer synthesis, in-house production is not recommendable anymore. However, in case of outsourcing, of course CELLFREE is your good choice.

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