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Product Details:
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| Method-1: | Antigen-specific Affinity Purification | Method-2: | Protein A/G Purification |
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| Option1: | Low Endotoxin Purification | Option2: | IgM Affinity Purification |
| Option3: | Ion-exchange Chromatography | Amount: | 100 Ml |
| High Light: | Ion Exchange Chromatography Antibody Discovery,Ion exchange Antibody Discovery |
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Introduction
CELLFREE offers a variety of methods for antibody purification from serum, ascites, and culture supernatants. We also offer antibody purification with low endotoxin levels for in-vivo use.
The main two methods for antibody purification, i.e. antigen-specific affinity purification and Protein A/G purification. The former method can purify antibodies that bind the antigen without regard to antibody class or isotype, and the other method can purify all antibodies of the target class without regard to antigen specificity. Both two method rely on using a substance which binds to a specific antibody, holding in place while the rest of the solution is washed away.
Antigen affinity
If a high purity of antibody is required, a target-specific peptide or recombinant protein affinity column can be used to obtain extremely pure antibodies. A minimum of 10 mg of pure antigen (or purified peptide) is required for the generation of custom resins to capture the target protein. Due to the highly specific purification process, the resulting antibody preparationtends to have lower background levels and lower non-specific binding rates than other purification methods.
Typically used for purification of polyclonal antibodies.
Includes custom column preparation & affinity purification (25mL) for the 1st run.
Antibody at >90% purity in your buffer of choice.
Protein A and Protein G
Protein A and Protein G are the most common choices for antibody purification due to their ability to bind the constant (Fc) region of IgG from various species. Most intact IgG antibodies can be purified efficiently by Protein A or Protein G, but their affinity differs by species and subclass of IgG.
(Maxi-scale: up to 100mg)
Antibody at >90% purity in your buffer of choice.(includes SDS-PAGE report)
Protein A/G Purification(Midi-scale: 1-5mg)
Antibody at >90% purity in your buffer of choice.(minimum 4 samples; includes SDS-PAGE report)
Protein A/G Purification
(Mini-scale: 100-400ug)
Antibody at >70% purity in your buffer of choice.(minimum 4 samples; includes SDS-PAGE report)
