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Lyophilized Powder SgRNA Library Construction RNA Guided Nuclease Library Crispr Cas Gene Editing

Lyophilized Powder SgRNA Library Construction RNA Guided Nuclease Library Crispr Cas Gene Editing
Lyophilized Powder SgRNA Library Construction RNA Guided Nuclease Library Crispr Cas Gene Editing

Large Image :  Lyophilized Powder SgRNA Library Construction RNA Guided Nuclease Library Crispr Cas Gene Editing

Product Details:

Brand Name: CELLFREE
Model Number: CF-GE-009

Payment & Shipping Terms:

Packaging Details: vial
Delivery Time: 2~4 weeks
Detailed Product Description
Material: Plasmid Appearance: Lyophilized Powder
Package: Vial QC: Sequence Report
Special Treatment: Lentivirus Packaging Extra Service: Screening Report
High Light:

Lyophilized Powder Crispr Cas Gene Editing

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RNA Guided Crispr Cas Gene Editing

Introduction

 

CRISPR-Cas9/gRNA (CRISPR-Cas RNA-Guided nuclease) is considered as the biggest breakthrough in genome editing technology after TALEN and ZFN. It precisely edits DNA through the RNA-directed Cas9 nuclease. Previous studies indicated that the on-target gene knockout rates were higher than that with TALEN and ZFN. Besides, the construction of sgRNA-Cas9 is easier and more convenient. It has been widely applied to animal and plant breeding, directed evolution, and the site-specific restoration of genetic diseases. CELLFREE offers the designing, construction and validation of CRISPR-Cas9 gene target system through our patent pending platform in a highly cost and time-effective approach. We offer one stop solution for CRISPR Cas9 projects, including genome scale gene targeting.

 

Service list

 

Service Type Service Specifications Deliverables
CRISPR-Cas9 sgRNA lentivirus library (Human) Targeting 21,000 genes; 6 sgRNAs for each gene Lentivirus, -80 ℃ preservation
Customized CRISPR-Cas9 sgRNA Library Customized gRNA library, including genome-scale library, lncRNA library, cell apoptosis, cell proliferation, signal pathway, ion channel, nuclear receptor libraries Lyophilized sgRNA plasmid Sequencing report
Lentivirus Packaging of sgRNA Library Customized sgRNA design, 6 sgRNA for each gene Lentivirus package Lentivirus
sgRNA Library Screening Cell screening using cell survival control, immunostaining, flow cytometry and other screening methods Screening Report and Sequencing Data
Service Type Service Specifications Deliverables
CRISPR-Cas9 sgRNA lentivirus library (Human) Targeting 21,000 genes; 6 sgRNAs for each gene Lentivirus, -80 ℃ preservation
Customized CRISPR-Cas9 sgRNA Library Customized gRNA library, including genome-scale library, lncRNA library, cell apoptosis, cell proliferation, signal pathway, ion channel, nuclear receptor libraries Lyophilized sgRNA plasmid Sequencing report
Lentivirus Packaging of sgRNA Library Customized sgRNA design, 6 sgRNA for each gene Lentivirus package Lentivirus
sgRNA Library Screening Cell screening using cell survival control, immunostaining, flow cytometry and other screening methods Screening Report and Sequencing Data

 

Advantages

 

  • Proprietary DNA synthesis platform: fast and efficient sgRNA library construction
  • Lentivirus transfection system: improve the efficiency of sgRNA transfection
  • Multiple screening platform: including cell survival control, immunostaining, flow cytometry and other screening methods
  • One-stop solution: customized services ranging from sgRNA library design, synthesis, and lentivirus packaging, to high throughput/content screening

 

sg RNA Library Construction and Screening Workflow

Lyophilized Powder SgRNA Library Construction RNA Guided Nuclease Library Crispr Cas Gene Editing 0

 

Contact Details
CELLFREE SCIENCE CO., LTD.

Contact Person: Pan Lin

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