Product Details:
Payment & Shipping Terms:
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| Material: | Plasmid | Appearance: | Lyophilized Powder |
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| Package: | Vial | QC: | Sequence Report |
| Special Treatment: | Lentivirus Packaging | Extra Service: | Screening Report |
| High Light: | Lyophilized Powder Crispr Cas Gene Editing,RNA Guided Crispr Cas Gene Editing |
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Introduction
CRISPR-Cas9/gRNA (CRISPR-Cas RNA-Guided nuclease) is considered as the biggest breakthrough in genome editing technology after TALEN and ZFN. It precisely edits DNA through the RNA-directed Cas9 nuclease. Previous studies indicated that the on-target gene knockout rates were higher than that with TALEN and ZFN. Besides, the construction of sgRNA-Cas9 is easier and more convenient. It has been widely applied to animal and plant breeding, directed evolution, and the site-specific restoration of genetic diseases. CELLFREE offers the designing, construction and validation of CRISPR-Cas9 gene target system through our patent pending platform in a highly cost and time-effective approach. We offer one stop solution for CRISPR Cas9 projects, including genome scale gene targeting.
Service list
| Service Type | Service Specifications | Deliverables |
| CRISPR-Cas9 sgRNA lentivirus library (Human) | Targeting 21,000 genes; 6 sgRNAs for each gene | Lentivirus, -80 ℃ preservation |
| Customized CRISPR-Cas9 sgRNA Library | Customized gRNA library, including genome-scale library, lncRNA library, cell apoptosis, cell proliferation, signal pathway, ion channel, nuclear receptor libraries | Lyophilized sgRNA plasmid Sequencing report |
| Lentivirus Packaging of sgRNA Library | Customized sgRNA design, 6 sgRNA for each gene Lentivirus package | Lentivirus |
| sgRNA Library Screening | Cell screening using cell survival control, immunostaining, flow cytometry and other screening methods | Screening Report and Sequencing Data |
| Service Type | Service Specifications | Deliverables |
| CRISPR-Cas9 sgRNA lentivirus library (Human) | Targeting 21,000 genes; 6 sgRNAs for each gene | Lentivirus, -80 ℃ preservation |
| Customized CRISPR-Cas9 sgRNA Library | Customized gRNA library, including genome-scale library, lncRNA library, cell apoptosis, cell proliferation, signal pathway, ion channel, nuclear receptor libraries | Lyophilized sgRNA plasmid Sequencing report |
| Lentivirus Packaging of sgRNA Library | Customized sgRNA design, 6 sgRNA for each gene Lentivirus package | Lentivirus |
| sgRNA Library Screening | Cell screening using cell survival control, immunostaining, flow cytometry and other screening methods | Screening Report and Sequencing Data |
Advantages
sg RNA Library Construction and Screening Workflow
