Puromycin Metabolic Labeling Protein Expression And Purification

Introduction

Biological research often requires the use of molecular labels that are covalently attached to a protein of interest to facilitate detection or purification of the labeled protein and/or its binding partners. Labeling strategies result in the covalent attachment of different molecules, including biotin, reporter enzymes, fluorophores and radioactive isotopes, to the target protein or nucleotide sequence. While multiple types of labels are available, their varied uses are preferable for specific applications. Therefore, the type of label and the labeling strategy used must be carefully considered and tailored for each application.

Our custom protein labeling & conjugation service provides personalized solutions designed to supply researchers with the widest choice of labels and conjugates to meet their specific needs . We offers high quality, efficient and cost-saving labeling & conjugation services with the largest selections of labels and protocols. We can help you decide on the most suitable conjugate or approach for your applications and optimize labeling procedures toward your specific need.

Services contents

• Stable Isotope Labeling
• Protein Biotinylation
• Fluorescent Labeling in Vitro
• Fluorescent Tagged Protein in Vivo
• Enzyme/Antibody conjugation

Application

• Biomolecular NMR

• Enzyme & Antibody modifications and conjugations (biotin, chromo- and fluorophores)

• Nanoparticle functionalization (e.g gold-labeled, magnetic nanoparticles, silica particle)

• Surface immobilization (e.g. Microtitre wells, silica and gold slides)

• Development of 'small' molecule drug & enzyme conjugates to therapeutic antibodies

• Modification of key properties of therapeutic proteins to optimise PK/PD behaviour

• In-vivo protein trafficking and subcellular localization

• Conjugated biopharmaceuticals for targeted delivery of cytotoxic agents