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|SiRNA/shRNA Functional Knockdown:||QRT-PCR And Western Blot||Transient:||SiRNA And ShRNA|
|Screens:||RNAi||PCR Cloning:||ShRNA-encoding Vector Construction|
qRT-PCR Rna Interference Gene Silencing,
Western Blot Rna Interference Gene Silencing
RNA Interference (RNAi) is a powerful tool used to effectively regulate gene expression and is critical in the drug discovery process. Knockdown of target genes with RNAi reagents can be used to facilitate the identification and validation of drug targets and to elucidate biochemical and cell signaling pathways. RNAi is a specific, potent, and highly successful approach for loss-of-function studies in virtually all eukaryotic organisms. RNAi also offers the promise of a new, faster, more cost-effective, and more versatile alternative to current transgenic mouse knockout technologies.
With roots as a pioneer of genome-scale RNAi screening and long-standing contributions towards refining best practices in this field, CELLFREE provides leading functional genomics technologies including RNA interference (RNAi) tools for gene silencing (siRNA, shRNA, miRNA) and cutting-edge systems for gene editing and gene knockout. All RNAi research projects carried out by CELLFREE are custom-designed to address each client's specific experimental, strategic and budgetary guidelines, while always meeting the highest scientific standards in the field. CELLFREE has developed a unique expertise of handling all steps involved from dsRNA design and production to fully automated screening and data analysis.
Our standard RNAi services include: