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qRT-PCR Western Blot ShRNA Encoding Vector Rna Interference Gene Silencing

qRT-PCR Western Blot ShRNA Encoding Vector Rna Interference Gene Silencing
qRT-PCR Western Blot ShRNA Encoding Vector Rna Interference Gene Silencing

Large Image :  qRT-PCR Western Blot ShRNA Encoding Vector Rna Interference Gene Silencing

Product Details:

Brand Name: CELLFREE
Model Number: CF-RN-010

Payment & Shipping Terms:

Packaging Details: vial
Delivery Time: 10 to 45 working days
Detailed Product Description
SiRNA/shRNA Functional Knockdown: QRT-PCR And Western Blot Transient: SiRNA And ShRNA
Screens: RNAi PCR Cloning: ShRNA-encoding Vector Construction
Electronic Data: Report Delivery: Plasmid
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qRT-PCR Rna Interference Gene Silencing

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Western Blot Rna Interference Gene Silencing

Introduction

 

RNA Interference (RNAi) is a powerful tool used to effectively regulate gene expression and is critical in the drug discovery process. Knockdown of target genes with RNAi reagents can be used to facilitate the identification and validation of drug targets and to elucidate biochemical and cell signaling pathways. RNAi is a specific, potent, and highly successful approach for loss-of-function studies in virtually all eukaryotic organisms. RNAi also offers the promise of a new, faster, more cost-effective, and more versatile alternative to current transgenic mouse knockout technologies.

 

With roots as a pioneer of genome-scale RNAi screening and long-standing contributions towards refining best practices in this field, CELLFREE provides leading functional genomics technologies including RNA interference (RNAi) tools for gene silencing (siRNA, shRNA, miRNA) and cutting-edge systems for gene editing and gene knockout. All RNAi research projects carried out by CELLFREE are custom-designed to address each client's specific experimental, strategic and budgetary guidelines, while always meeting the highest scientific standards in the field. CELLFREE has developed a unique expertise of handling all steps involved from dsRNA design and production to fully automated screening and data analysis.

 

Service content

 

Our standard RNAi services include:

  • In vivo siRNA delivery
  • Quantitation of siRNA/shRNA functional knockdown effect by qRT-PCR and Western Blot
  • siRNA transfection optimization
  • Generation of stable RNAi cell lines (stably expressing shRNA targeting gene of interest)
  • Transient (siRNA) and stable (shRNA) transfection services
  • siRNA, shRNA, miRNA design and synthesis
  • Altogen’s tetracycline-inducible RNAi systems
  • RNAi Screens
  • Genome-wide siRNA and microRNA high-throughput screens
  • Gene targeting
  • RNAi reporter cell lines generation
  • In vitro functional testing of siRNA-induced mRNA knockdown
  • RNAi screen pathway analysis
  • RNAi manufacturing
  • siRNA and microRNA chemical modification to enhance stability in vivo
  • PCR cloning: shRNA-encoding vector construction
  • Reporter gene (RNAi) assays

Contact Details
CELLFREE SCIENCE CO., LTD.

Contact Person: Pan Lin

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