Rnai Vector Construction Rna Interference Expression Vectors Nucleolus Rna Synthesis

Introduction

RNA interference (RNAi) is a process that has been found in all living cells. This mechanism is responsible for inhibiting the expression level or further behavior or development of specific genes. In turn, this inhibition affects the duplication of target genes or results in a lack of certain proteins. RNAi technologies can be exploited for the treatment of diseases through the effective silencing of genes and expression of genes (e.g. oncogenes) that cause diseases.

RNAi allows researchers to fully or partially suppress the expression of a specific gene, allowing targeted gene knockout and gene knockdown.

Small interfering RNAs, or siRNAs, are short RNA molecules of 19 to 22 nucleotides in length. The siRNAs are generated via cleavage of dsRNA templates by DICER, an RNAse III ribonuclease. The siRNAs are then incorporated into an RNA-induced silencing complex (RISC) and unwound into single-stranded siRNAs. Next, the single-stranded siRNAs guide the RISC complex to the target mRNAs for destruction, causing RNA interference. Depending on the amount of siRNA expressed and its inhibitory efficiency, expression of the target gene can be either completely blocked or measurably suppressed. This allows researchers to determine and study the function of genes, particularly the genes that are lethal upon complete knockout. CELLFREE's siRNA expression vectors provide the fine degree of controlled RNAi necessary to these delicate experiments.

CELLFREE pays attention to the latest drug development programs by endogenous RNAi machinery and offers a total solution for RNAi therapy discovery and development, from RNA design, synthesis, profiling, in vitro screening, in vivo validation.

Service content

We offer custom RNAi services for your scientific research as follows:

• siRNA, shRNA and miRNA design and synthesis: customer provides target gene name, sequence or GeneBank ID and so on.
• Choice of shRNA and miRNA vector.
• siRNA, shRNA and miRNA transfection optimization service.
• Test of inhibitory effect: the mRNA expression is detected by RT- PCR; the protein expression is detected by Western Blot.
• Provision of experimental reports RNAi sequence, RT-PCR test report, Western Blot picture and so on.
• Custom preparation of shRNA and miRNA expressing lentiviral particles, determination of the virus titer and establishment of stable cell lines.