Product Details:
Payment & Shipping Terms:
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| Material: | Engineered Strain | Package: | Glycerol Stock |
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| QC: | COA | Electronical Data: | Sequence Chromatogram |
| Object: | E. Coli | Validation: | Quantitative RT-PCR |
| High Light: | Actinomyces Crispr Cas Gene Editing,RT-PCR Crispr Cas Gene Editing |
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Introduction
Microbes are widely used in pharmaceutical, pesticides, food industry, energy and a series of innovative applications. Through the research on microbe genome, functional genes related with new special enzyme, important metabolic processes and metabolite are constantly being discovered and applied to production as well as the transformation of traditional industry and technology. It has a certain value in the application of bio-pharmaceutics, pollution control, energy and chemical, biological manufacturing industries. Early strain transformation mainly concentrate on random screening and simple rational screening, but the disadvantages which included time-consuming, laborious, heavy workload and no orientation of mutagenesis in traditional methods were gradually exposed as time went on. With the development of modern molecular biology techniques, many new methods for strains breeding appeared with directionality and positive mutation. The emergence of CRISPR-Cas9 technology has greatly promoted the accuracy and efficiency of microbe genome editing.
Competitive Advantages
Operation Process in E.coli
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Service |
E. coli Knock-out |
E. coli Knock-in |
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| Guide RNA Design | Our expert scientists will optimize your guide RNA sequence for maximized efficiency and minimal off-target effects using design algorithms created at the Broad Institute of MIT and Harvard. | |
| Materials Needed from Clients |
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| Multiplex Editing | Up to 3 targets | Single knock-in/replacement |
| Deliverables | Engineered strain in glycerol stock | |
| Quality Control |
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| Delivery Time | Starting from 4 weeks | |
Flow chart
