ShRNA Vector RNAi Vector Construction Nucleolus Rna Synthesis

Introduction

In molecular cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where it can be replicated and/or expressed (e.g.- plasmid, cosmid, Lambda phages). The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes. Among these, the most commonly used vectors are plasmids. Nowadays, a wide variety of plasmid vectors are commercially available. However, in some cases, the commercial vectors cannot best match researcher’s specific needs and researchers have to rely on in house vector modification.

CELLFREE is able to provide custom vector construction and modification service to generate custom specialized plasmid vectors with distinct characteristics quickly and reliably. We have developed an advanced gene synthesis platform to modular construct custom plasmid vectors. We obtain the vector modules either by PCR amplification or synthetical methods and using complex cloning strategies when needed to develop completely customized vectors to best fit your unique requirements. Our goal is to provide you with the most affordable, high-quality custom vector construction and modification service to ensure your satisfaction in a timely and professional manner.

Services content

• Cell-based shRNA services: including shRNA design, shRNA construction, shRNA transfection, and function characterization
• Lentivirus-based shRNA services: including vector construction, lentivirus particle packaging, RNAi validation, and stable cell lines construction
• Adenovirus-based shRNA services: including vector construction, adenovirus particle packaging, and RNAi validation
• Stem cell-based shRNA services
• RNAi effects in stem cells

Delivery Form

• Lyophilized plasmid DNA (2~5 µg)
• Sequencing chromatogram
• Certificate of analysis (COA)
• Virus particle (Optional)
• Cell line (Optional)