Product Details:
Payment & Shipping Terms:
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Starting Material: | GDNA (extractions Available) | Assay Coverage: | Annotated SNPs Of Interest |
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Confirm: | SNPs | Deliverables: | Raw Data Files |
Reports: | Nucleotide Level Mutations | Turnaround: | A Week |
High Light: | SNP Genotying Molecular Genetic Analysis,Single Nucleotide Polymorphism Molecular Genetic Analysis |
Introduction
Genotyping is a method used to determine the genetic makeup of an organism. This method identifies genetic variations by comparing an individual’s sequence against a wild-type reference sequence to analyze single nucleotide polymorphisms (SNPs). SNPs are present in both the coding and non-coding regions of a gene and are one of the most common types of variants; they are represented as a single nucleotide mutation within a DNA sequence.
Whether you are using genotyping as your primary approach for SNP analysis and detection, or to confirm next generation sequencing and microarray results, CELLFREE can help accelerate your project. Let the Sanger sequencing experts at CELLFREE support your SNP genotyping research objectives with speed, quality, and reliability.
SNP genotyping platforms
Direct Sequencing
Restricted Fragment Length Polymorphisms (RFLP)
TaqMan Probe
Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)
SNP GENOTYPING WORKFLOW
1. Project consultation & primer design
amplicon selection and primer design to targeted snps of interest
2. Assay development
optimization of project-specific samples
3. Pcr & purification
pcr amplification of genomic dna using optimized conditions
4. Sequencing & data analysis
Bi-directional DNA sequencing per amplicon and final report identifying SNPs
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