Single Nucleotide Polymorphism SNP Genotying Molecular Genetic Analysis

Introduction

Genotyping is a method used to determine the genetic makeup of an organism. This method identifies genetic variations by comparing an individual’s sequence against a wild-type reference sequence to analyze single nucleotide polymorphisms (SNPs). SNPs are present in both the coding and non-coding regions of a gene and are one of the most common types of variants; they are represented as a single nucleotide mutation within a DNA sequence.

Whether you are using genotyping as your primary approach for SNP analysis and detection, or to confirm next generation sequencing and microarray results, CELLFREE can help accelerate your project. Let the Sanger sequencing experts at CELLFREE support your SNP genotyping research objectives with speed, quality, and reliability.

SNP genotyping platforms

1. Direct Sequencing

2. Restricted Fragment Length Polymorphisms (RFLP)

3. TaqMan Probe

4. Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)

SNP GENOTYPING WORKFLOW

       1. Project consultation & primer design

       amplicon selection and primer design to targeted snps of interest

2. Assay development
optimization of project-specific samples

3. Pcr & purification
pcr amplification of genomic dna using optimized conditions

4. Sequencing & data analysis
Bi-directional DNA sequencing per amplicon and final report identifying SNPs

Service content

• Consultation of assay design, such as the selection of site.
• DNA samples QA and standardization.
• Design, synthesis and optimization of primers used for genotyping.
• Quality evaluate of genotyping data.

Customer offer

• SNP sites information: SNP sites RS number or 200bp exact sequence upstream and downstream and the mutation type of SNP sites.
• Genotyping samples: genomic DNA, plasmid DNA, blood spots and so on

We deliver

• SNPs results (in excel file)
• primer and probe sequence used in reaction system
• primary genotyping result
• quality assessment report