Fragment gene synthesis are double-stranded, linear DNA fragments of 100-3,000 bp, which are a convenient option due to their faster delivery time, lower cost, and greater flexibility. These fragments are synthesized with the same industry-leading proprietary technology that CELLFREE has developed for standard gene synthesis products. We provide Fragment gene synthesis in a lyophilized form that can be easily re-suspended, cloned, and screened to identify the correct clone for downstream applications.

FEATURES

1. Highly reliable: Researchers at leading pharma, biotech, and academic institutions rely on CELLFREE for their synthetic DNA library needs

2. Improved accuracy: More precise control over library diversity allows you to overcome the inefficiencies of traditional approaches

3. Convenience: Whether you need a high-diversity synthetic DNA library or a small rationally-designed variant library, CELLFREE can cater to all your DNA library needs, no matter the complexity or size.

4. High diversity: Achieve library diversity of 10⁹ to 10¹² depending on the type of project deliverable

5. Quality control: Rigorous quality checks include statistical analysis and sequencing to ensure library diversity and quality

Deliverables

Standard deliverables include 500-1,000 ng of lyophilized double-stranded DNA, depending on sequence length.

• 400 ng lyophilized double-stranded DNA
• Quality Assurance Certificate: Gel Analysis

QUALITY CONTROL PROCEDURE

• Size verification by gel electrophoresis

• Sequence confirmation via Sanger sequencing

• Sequence identification by mass spectrometry

APPLICATIONS

• Assemble multiple gene fragments to make full-length gene constructs for protein expression and purification

• gRNA expression cassettes for CRISPR/Cas9-based gene editing

• Donor constructs for gene editing experiments

• Template for in vitro transcription

Typical Gene Synthesis Workflow

1. Oligonucleotide synthesis

2. Gene assembly

3. Quality control

4. Synthetic gene shipment