Payment & Shipping Terms:
|Material:||Nucleic Acid||Product:||Lyophilized Double-stranded DNA|
Lyophilized Double Stranded DNA Fragment Synthesis,
Lyophilized Double Stranded DNA Synthesis
Fragment gene synthesis are double-stranded, linear DNA fragments of 100-3,000 bp, which are a convenient option due to their faster delivery time, lower cost, and greater flexibility. These fragments are synthesized with the same industry-leading proprietary technology that CELLFREE has developed for standard gene synthesis products. We provide Fragment gene synthesis in a lyophilized form that can be easily re-suspended, cloned, and screened to identify the correct clone for downstream applications.
Highly reliable: Researchers at leading pharma, biotech, and academic institutions rely on CELLFREE for their synthetic DNA library needs
Improved accuracy: More precise control over library diversity allows you to overcome the inefficiencies of traditional approaches
High diversity: Achieve library diversity of 109 to 1012 depending on the type of project deliverable
Quality control: Rigorous quality checks include statistical analysis and sequencing to ensure library diversity and quality
|Sequence Length*||Quantity||Completion Time**||Predicted Cloning Accuracy||Sequence Fidelity|
|100-750 bp||500 ng||3-5 business days||>90%||100%|
|750-1,000 bp||1,000 ng|
|1,001-2,000 bp||5-8 business days|
*Only sequences with overall GC content between 20% and 80% qualify for this service. GC/AT rich or sequences with complex repeats do not qualify.
**Orders containing more than 50 fragments will require additional turnaround time; Shipping time is expected to add 1-2 business days to completion time.
Standard deliverables include 500-1,000 ng of lyophilized double-stranded DNA, depending on sequence length.
QUALITY CONTROL PROCEDURE
Size verification by gel electrophoresis
Sequence confirmation via Sanger sequencing
Sequence identification by mass spectrometry
Assemble multiple gene fragments to make full-length gene constructs for protein expression and purification
gRNA expression cassettes for CRISPR/Cas9-based gene editing
Donor constructs for gene editing experiments
Template for in vitro transcription
Typical Gene Synthesis Workflow
Synthetic gene shipment