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Lyophilized Double Stranded DNA Fragment Synthesis

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Lyophilized Double Stranded DNA Fragment Synthesis

Lyophilized Double Stranded DNA Fragment Synthesis
Lyophilized Double Stranded DNA Fragment Synthesis

Large Image :  Lyophilized Double Stranded DNA Fragment Synthesis

Product Details:

Brand Name: CELLFREE
Model Number: CF-ES-003

Payment & Shipping Terms:

Packaging Details: vial
Delivery Time: 5 days
Detailed Product Description
Material: Nucleic Acid Product: Lyophilized Double-stranded DNA
Report: COA Service: Fragments Synthesis
Quantity: 500-1,000 Ng Package: Vial
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Lyophilized Double Stranded DNA Fragment Synthesis


Lyophilized Double Stranded DNA Synthesis

Fragment gene synthesis are double-stranded, linear DNA fragments of 100-3,000 bp, which are a convenient option due to their faster delivery time, lower cost, and greater flexibility. These fragments are synthesized with the same industry-leading proprietary technology that CELLFREE has developed for standard gene synthesis products. We provide Fragment gene synthesis in a lyophilized form that can be easily re-suspended, cloned, and screened to identify the correct clone for downstream applications.



  1. Highly reliable: Researchers at leading pharma, biotech, and academic institutions rely on CELLFREE for their synthetic DNA library needs

  2. Improved accuracy: More precise control over library diversity allows you to overcome the inefficiencies of traditional approaches

  3. Convenience: Whether you need a high-diversity synthetic DNA library or a small rationally-designed variant library, CELLFREE can cater to all your DNA library needs, no matter the complexity or size.
  4. High diversity: Achieve library diversity of 109 to 1012 depending on the type of project deliverable

  5. Quality control: Rigorous quality checks include statistical analysis and sequencing to ensure library diversity and quality


Sequence Length* Quantity Completion Time** Predicted Cloning Accuracy Sequence Fidelity
100-750 bp 500 ng 3-5 business days >90% 100%
750-1,000 bp 1,000 ng
1,001-2,000 bp 5-8 business days
2,001-3,000 bp

*Only sequences with overall GC content between 20% and 80% qualify for this service. GC/AT rich or sequences with complex repeats do not qualify.
**Orders containing more than 50 fragments will require additional turnaround time; Shipping time is expected to add 1-2 business days to completion time.




Standard deliverables include 500-1,000 ng of lyophilized double-stranded DNA, depending on sequence length.

  • 400 ng lyophilized double-stranded DNA
  • Quality Assurance Certificate: Gel Analysis


  • Size verification by gel electrophoresis

  • Sequence confirmation via Sanger sequencing

  • Sequence identification by mass spectrometry


  • Assemble multiple gene fragments to make full-length gene constructs for protein expression and purification

  • gRNA expression cassettes for CRISPR/Cas9-based gene editing

  • Donor constructs for gene editing experiments

  • Template for in vitro transcription

Typical Gene Synthesis Workflow

  1. Oligonucleotide synthesis

  2. Gene assembly

  3. Quality control

  4. Synthetic gene shipment




Contact Details

Contact Person: Pan Lin

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