Site Directed Mutation service DNA mutations multiple mutations study protein function

Introduction

Site-directed mutagenesis facilitates life science research by creating specific DNA mutations (including point mutations, deletion mutations, and insertion mutations) to enable studies of gene regulatory elements, DNA-protein interactions, protein structure/function, enzyme active sites, and novel proteins.

CELLFREE site-directed mutagenesis services provide a fast, cost-saving, way to get 100% accurate mutant DNA clones, without having to spend your time and money on mutagenesis kits to do PCR mutagenesis, cloning, and sequencing in your own lab. Powered by our industry-leading gene synthesis technology and high-performance GS polymerase, we can make any gene template and any number of mutations, starting at just 5-7 day turnaround time.

Benefits

1. 100% accuracy: mutagenized genes are sequence-verified via Sanger sequencing as per your requirements

2. Full sequence-confirmed mutants delivered in as little as 5 working days

3. Multiple mutations in one reaction; successful large deletion/insertion protocol

4. Optimized, proprietary CELLFREE protocol for greater reliability

Application

• Study protein function, structure-function relationships, or nucleic acid-protein interactions
• Identify active sites or functional elements such as promoter regions
• Modify regulatory sequences to alter gene expression
• Construct fusion, tagged proteins, or knockouts

What we need

Gene information, mutation site sequence information, wild type plasmid or bacterial solution, plasmid ≥ 2 μg, wild type plasmid sequencing map, vector map, related literature, etc.

The fastest, and usually the most cost-effective, way to get the mutant DNA constructs you need is to order Express Mutagenesis bundled with gene synthesis.

Delivery Specifications for Mutagenesis Services

All of our mutagenesis services offer the following delivery package:

1. 4 μg lyophilized destination vector containing the gene

2. Sequence chromatograms covering your gene (electronic)

3. Construct map for the plasmid (electronic)

4. Quality assurance certificate