Product Details:
Payment & Shipping Terms:
|
| Material: | Lyophilized Plasmid DNA (2~5 µg) | Document: | Sequencing Chromatogram & COA |
|---|---|---|---|
| Turnaround Time: | 5~30 Days | Data: | Gel Analysis Photos Of PCR |
| • DNA Sequence: | Available | Starting Information: | Electronic Sequence For Gene Synthesis |
| High Light: | 2µg DNA Cellular Molecular Biology,COA Cellular Molecular Biology |
||
We, CELLFREE, offers cloning services so you can free yourself from routine gene cloning and instead devote your energy and time to more creative research. Our molecular biology experts can bundle gene synthesis with cloning into your choice of vector, or you can outsource DNA cloning projects from templates you already have.
PCR cloning strategies
PCR cloning is a method in which double-stranded DNA fragments amplified by PCR are ligated directly into a vector. PCR cloning offers some advantages over traditional cloning which relies on digesting double-stranded DNA inserts with restriction enzymes to create compatible ends, purifying and isolating sufficient amounts, and ligating into a similarly treated vector of choice.
Subcloning basics
Subcloning refers to moving one fragment of a plasmid into another plasmid that can serve as a vector. There are a variety of reasons why it is necessary to transfer the fragment of interest into a different vector backbone. For instance, the new vector may possess a specific marker for antibiotic selection or fluorescent expression. Subcloning may also be performed to move a cloned fragment to an expression vector of a more suitable host for the study (e.g., bacteria, mammals, insects, plants, etc.); to place the gene of interest under a different expression promoter (e.g., a constitutive to inducible promoter); or to tag or fuse the experimental gene with another protein or a marker. Whatever the goal of the experiment may be, the two most common approaches to subcloning rely on restriction digestion and/or PCR cloning.
PCR Cloning & Subcloning Related services
We synthesize the error-free gene sequences on the basis of our proprietary synthesis platform.
High efficiency plasmid extraction can result in research-level or preclinical level plasmid prep to meet multiple demands.
Based on our gene synthesis and next generation DNA synthesis platforms, CELLFREE will deliver any synthetic vector you request with high accuracy and at an economical price.
Advantages
