DNA Libraries Mutagenesis Library Gene Library Biobasic Gene Synthesis

Synthetic DNA Libraries Services Mutagenesis Library And Gene Library Synthesis

Gene library refers to a collection of large numbers of long DNA sequences (> 200bp). These sequences can be related or unrelated. Libraries of related sequences include sequence variants or mutant library, codon variants library, amino acid sequence variants library, regulatory sequence combination library, etc. Libraries of unrelated sequences can include any DNA sequences. We can help customers to design and synthesize any type of gene libraries according to customer’s specifications. These libraries can be cloned into a vector or exist as linear double-stranded DNAs.

 Featured Synthetic Dna Libraries

1. Alanine Scanning Library: Alanine scanning is a widely-used approach that involves substituting non-alanine residues sequentially with alanine within a customer-determined region by site-directed mutagenesis. Cysteine scanning is another technique that researchers use for certain studies.

2. Combinatorial Library: A combinatorial library allows you to create a library of mutants by using different genetic elements in multiple combinations to determine the most beneficial combination. You can obtain a combinatorial library using traditional partially randomized (NNK/NNS) or completely randomized (NNN) approaches.

3. Randomized Library: A randomized mutant library will allow you to create random amino acid substitutions in a small user-defined region such as an ORF. These proteins can then be expressed and assayed for function to determine beneficial substitutions in protein engineering studies.

4. Site-directed Mutagenesis Library: In a site-directed mutagenesis library, a customer-determined amino acid position will be substituted sequentially with all the other 19 naturally-occurring amino acids to create a library of mutants. With this approach, you can use structural-functional analysis to examine the benefits or adverse effects of a particular amino acid substitution.

5. Truncation Library: A truncation library of mutants is created by trimming amino acids systematically from either the N- or C-terminus (or both), of a protein around a pre-defined core region. This allows you to identify the core region responsible for protein function.

Available options for project deliverables

Certain libraries may only be available as individual clones.

Applictions

• Protein Engineering: Build and identify proteins with novel or improved stability and functional characteristics from a library of mutants
• Enzyme Engineering: Modify an enzyme for enhanced activity for use in industrial biotechnology
• Directed Evolution: Build a library of variants with your desired mutations for applications in evolutionary biology
• Synthetic Biology: Build new metabolic pathways with different combinations of genetic elements for applications in metabolic engineering
• Antibody Development: Test and identify mutant sequences with improved antigen expression

Typical DNA Libraries Synthesis Workflow