Home ProductsBiobasic Gene SynthesisSsDNA COA Synthesis Gene Synthesis Single Stranded DNA Synthesis
I'm Online Chat Now

SsDNA COA Synthesis Gene Synthesis Single Stranded DNA Synthesis

SsDNA COA Synthesis Gene Synthesis Single Stranded DNA Synthesis
SsDNA COA Synthesis Gene Synthesis Single Stranded DNA Synthesis

Large Image :  SsDNA COA Synthesis Gene Synthesis Single Stranded DNA Synthesis

Product Details:

Brand Name: CELLFREE
Model Number: CF-ES-005

Payment & Shipping Terms:

Packaging Details: vial
Delivery Time: 25 days
Detailed Product Description
Material: Nucleic Acid Product: Lyophilized DNA
Package: Vial Service: Single-stranded
Document: COA Electronic Information: Sequence Files
High Light:

SsDNA COA Single Stranded DNA Synthesis


Lyophilized DNA Single Stranded DNA Synthesis

SsDNA Synthesis Services Single Stranded DNA Synthesis Gene Synthesis

Recent research in CRISPR/Cas9 technology has shown that the use of long single-stranded DNA (ssDNA) donor templates greatly enhances the efficiency of homology directed repair (HDR) enabling researchers to optimize the process of efficiently generating transgenic animal models and cell lines.


CELLFREE's ssDNA synthesis service provides up to 5000 nt of full sequence-verified fragments quickly and affordably. Our fragments are derived from clonally purified double-stranded DNA (dsDNA), producing the highest quality results possible. We offer services of sequence-verified ssDNA synthesis for CRISPR-mediated gene knock-in, in-vitro transcription, and much more.



  1. Lower cellular toxicity compared to dsDNA after cellular delivery

  2. Low off-target integration for more reliable gene knock-ins

  3. High specificity knock-in templates for homology directed repair

  4. High efficiency donors for targeted insertions and gene replacements



Double-stranded breaks are generated through CRISPR/Cas9 editing, then repaired by the endogenous cellular pathways of non-homologous end joining (NHEJ) and HDR. While the HDR pathway has consistently proven successful in copying genetic information via homologous recombination, insertion of exogenous genetic material is a challenge due to the inherent inefficiencies of HDR. Double-stranded DNA has historically been the template of choice for gene insertions, but recent research has shown the superiority of ssODNs as a donor template for HDR. Offering much higher efficiency to insert long sequences with shorter homology arms, ssDNA has become the preferred donor template for this process.


SsDNA COA Synthesis Gene Synthesis Single Stranded DNA Synthesis 0


  1. Antibody Discovery: Engineer customized cell lines or transgenic mouse models to study in vivo immune responses

  2. Food technology: Modify genomes of agricultural crops to study pathogenic resistance for improved food security

  3. Cancer Biology: Generate CRISPR gene insertions to study oncogene function for targeted therapeutics

  4. Biofuels:  Precise genome editing to optimize metabolic pathways for biofuel production



Length Quantity (Lyophilized) Turnaround Time
151-500 nt 3µg or 10 µg or 20 µg 15-20 business days
501-3000 nt 20-25 business days
3001-5000 nt 25-30 business days



  1. Sequence verified
  2. 3 µg, 10 µg, or 20 µg lyophilized DNA
  3. Certificate of Analysis (COA) including gel image, sequence trace data with alignment, and sequence files

Contact Details

Contact Person: Pan Lin

Send your inquiry directly to us (0 / 3000)