4 nmol Lyophilized Powder SgRNAs Service Crispr Cas Gene Editing

Introduction

CRISPR/Cas9 technology is a powerful tool for creating gene knock-ins and knock-outs. Using the ribonucleoprotein (RNP) system for delivering CRISPR/Cas9 has many advantages over the traditional plasmids or virus based delivery methods.

The CRISPR-Cas9 system is a prokaryotic immune system driven by RNA that triggers resistance to foreign genetic elements and provides a form of acquired immunity. sgRNA serves as a guide to recognize and combine target with DNAs, allowing activated Cas9 nuclease to attach, cleave, and modify the PAM downstream target DNA. CRISPR-based genome editing technology has made it easier and faster than ever to alter specific DNA sequence in the genome or to perform genome-wide functional screening tests to identify genes involved in a particular phenotype. CRISPR-Cas9 is considered as the most promising tool in gene modification.
 

Advantages

• One-stop solution: CELLFREE provides integrated services from sgRNA target design to high purity ready-to-use sgRNA production. We also provide single sgRNA, multiply sgRNA and sgRNA library design service.
• Fast delivery: In just 3 business days, CELLFREE will deliver up to 20μg of customized ready-to-use sgRNA
• Convenience: ready-to-use sgRNA can be directly injected into animals or transfected into cells, improving the efficiency of gene editing experiments. Database covers sgRNA design for >20 different species; Specific sgRNA design is also available upon request for certain species.
• Custom Vector Construction: sgRNA can be designed in any vector that customers require.

Deliverables

Offer modified sgRNAs for better stability and editing efficiency

RNase-free HPLC guaranteeing more than 90% purity

97-103nt length range, allowing for greater flexibility in design

ESI-MS guaranteeing mass variation less than 1‰

4 nmol guaranteed delivery quantity

*Deliver in as short as 8 business days.