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E. coli Stocks ShRNA Vector A Target Gene Vector Crispr Cas Gene Editing

E. coli Stocks ShRNA Vector A Target Gene Vector Crispr Cas Gene Editing
E. coli Stocks ShRNA Vector A Target Gene Vector Crispr Cas Gene Editing

Large Image :  E. coli Stocks ShRNA Vector A Target Gene Vector Crispr Cas Gene Editing

Product Details:

Brand Name: CELLFREE
Model Number: CF-GE-011

Payment & Shipping Terms:

Packaging Details: vial
Delivery Time: 2~3 weeks
Detailed Product Description
Appearance: E. Coli Stocks Material: Plasmid
QC: DNA Quantification Turnaround Time: 2~3 Weeks
Extra Service: Non-standard Vectors Available Electronical Data: Sequencing Information
High Light:

shRNA vector Crispr Cas Gene Editing

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E. coli Stocks Vector Crispr Cas Editing

Introduction

 

RNA interference (RNAi) has become a powerful means for silencing target gene expression in mammalian cells and is envisioned to be useful in therapeutic approaches to human disease.

RNA interference (RNAi) blocks transcription and translation and inhibits gene expression by introducing double stranded RNA. RNAi is a quick, convenient, and effective method which is widely used to down-regulate gene expression. Using shRNA to silence a target gene of choice, RNAi is immensely useful in disease studies of viruses, tumors, and genetic disorders.

 

The siRNA expression vector with antibiotics labeling can continuously suppress the target gene expression, lasting for several weeks or even longer.
When a validated siRNA sequence (for example, obtained by screening in siRNA synthesis method) needs to maintain gene silencing for a longer period of time, we recommend the use of shRNA vector system.

 

We can help you to insert the DNA fragment encoding target shRNA into the plasmid and do sequence validation. You just need to tell us the target gene sequence or Gene ID and the name of the inserted vector, and we will help you to construct the expression vector. Furthermore, we will provide you with sufficient purified expression plasmid encoding your target shRNA. 

 

Services list

  • Cell-based shRNA services: including shRNA design, shRNA construction, shRNA transfection, and function characterization
  • Lentivirus-based shRNA services: including vector construction, lentivirus particle packaging, RNAi validation, and stable cell lines construction
  • Adenovirus-based shRNA services: including vector construction, adenovirus particle packaging, and RNAi validation
  • Stem cell-based shRNA services
  • RNAi effects in stem cells

 

Feather

  1. The method is simple, economical, and only needs to synthesize DNA oligos.
  2. The use of cloning vector is convenient, and the vector has selectable markers.
  3. shRNA plasmid is stable and easy to operate.

 

Delivery Form

  • Lyophilized plasmid DNA (2~5 µg)
  • Sequencing chromatogram
  • Certificate of analysis (COA)
  • Virus particle (Optional)
  • Cell line (Optional)

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Contact Details
CELLFREE SCIENCE CO., LTD.

Contact Person: Pan Lin

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