Payment & Shipping Terms:
|Material:||DNA Plasmid||Research Grade:||Endotoxin N/A|
|Industrial Grade:||Endotoxin<0.005EU/ug||Starting Material:||DNA; Bacterial Colony;Fresh Colonies|
0.005EU/ug Plasmid DNA Preparation,
Endotoxin Level Plasmid DNA Preparation
CELLFREE ’s plasmid DNA preparation service, customized to your desired quality and quantity needs, offers the most stringent quality control standards on the market.
Endotoxins can have adverse effects on certain downstream applications following plasmid purification. If found endotoxins are found at high enough levels, they can influence the process of transfection of DNA into cells, leading to reduced efficiencies.
Endotoxins can also have adverse effects on gene therapy applications, as they can lead to several side effects such as fever in both animals and humans and can lead to misinterpretation of research results. So we should care about endotoxins in plasmid preps.
CELLFREE offer low Endotoxin Level plasmid.
Transfection in mammalian cells
Protein and antibody production
DNA vaccine research
Gene and cell therapy studies
What we need
Starting material can be DNA (at least 100 ng in water or TE); Bacterial Colony: Fresh Colonies (<3 days old).
Glycerol Stock: 200 µl frozen stock
*If your DNA requires special hosts, please provide the bacterial colony or glycerol stock as starting material.Please contact us with any questions or for additional shipping instructions.
|Appearance||Clear, no visible particles||Visual inspection|
|A260/280||1.8-2.0||Nanodrop UV absorption|
|Concentration||Default 1 mg/ml; Specific concentration provided upon request||UV absorption|
|Restriction Analysis||Conforming to reference pattern||Enzyme digestion and electrophoresis|