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Codon Optimization DNA Sequences Protein Expression And Purification

Large Image : Codon Optimization DNA Sequences Protein Expression And Purification

Product Details:

Brand Name:	CELLFREE
Model Number:	CF-PR-012

Payment & Shipping Terms:

Packaging Details:	vial
Delivery Time:	2~5 working days

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Detailed Product Description

Customer Off-1er:	Target Sequence	Customer Offer-2:	Desired Number Of Constructs
Report:	DNA Sequences, Protein Expression Screening Analysis	Constructs:	Recommended
Optional-1:	Downstream Protein Expression	Optional-2:	Stable Cell Line Development
High Light:	Codon Optimization Protein Expression Purification , DNA Sequences Protein Expression Purification

Codon optimization is a novel technique to improve protein expression level in living organism by increasing translational efficiency of target gene. CELLFREE provides state-of-the-art algorithms to optimize gene sequences using in-house pre-computed software from a predicted group of highly expressed genes from thousands of samples.

The demand for recombinant proteins has increased significantly during the past decades, this has in turn increased the need for producing sufficient amounts of high-quality protein samples for various applications. CELLFREE codon optimization and verification services are designed to facilitate the identification of optimal genetic combinations for enhancing recombinant protein expression.

Applications

Express a novel protein target
Increase production level of a poorly expressed protein
Improve antibody production for downstream process development
Enhance protein expression level to reduce production cost

Key Features

Significant improvement in expression: Combinatorial approach for success
Comprehensive coverage: Optimization for bacteria, yeast, mammalian and plant system
Guaranteed result: Full report delivered for each combination tested

Service Specifications

Customer Provides	Service Specifications	Deliverables
• Target sequence (or accession number) to be optimized for Expression system to be optimized for: E. coli, Pichia pastoris, mammalian cells (CHO/HEK293), insect cells (sf9), green algae • Desired number of constructs/sequences to be evaluated	1. Construct optimization*: Sequence Optimization; Gene Synthesis 2. Subcloning to expression vector(s) 3. Experimental verification: Protein expression screening; SDS PAGE analysis; Test affinity purification when applicable	• Full report including optimization of DNA sequences, protein expression screening analysis and our recommended constructs • >2µg DNA of expression optimized construct with sequence file • Professional consultation on data analysis • (Optional) Downstream protein expression/purification, scale up production, stable cell line development services are also available upon request