Product Details:
Payment & Shipping Terms:
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| Starting With: | SNP Sites Information | Raw Material: | Genotyping Samples |
|---|---|---|---|
| SNPs Results: | Available | DNA Samples: | Deliverable |
| Primer And Probe Sequence: | Available | QA Report: | Available |
| High Light: | SNP Sites Information Cellular Molecular Biology,Polymorphism Genotying Cellular Molecular Biology |
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Introduction
Genotyping is a method used to determine the genetic makeup of an organism. This method identifies genetic variations by comparing an individual’s sequence against a wild-type reference sequence to analyze single nucleotide polymorphisms (SNPs). SNPs are present in both the coding and non-coding regions of a gene and are one of the most common types of variants; they are represented as a single nucleotide mutation within a DNA sequence.
CELLFREE have developed several SNP genotyping platforms for different requirement including:
Direct Sequencing
Restricted Fragment Length Polymorphisms (RFLP)
TaqMan Probe
Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)
WORKFLOW
1. Project consultation & primer design
Amplicon selection and primer design to targeted snps of interest
2. Assay development
Optimization of project-specific samples
3. Pcr & purification
Pcr amplification of genomic dna using optimized conditions
4. Sequencing & data analysis
Bi-directional DNA sequencing per amplicon and final report identifying SNPs
| Steps | Content |
| DNA extraction | Whole blood samples |
| Paraffin sections, tissue samples | |
| Pre-test | optimize PCR reaction conditions |
| PCR reaction | According to preliminary results of a large number of PCR |
| Label primers | Synthesis of fluorescent labeled primers |
| Formal test | STR genotyping using ABI 3730XL DNA analyzer |
| Requirements for samples | Genomic DNA |
| Whole blood samples | |
| Paraffin sections, tissue samples |
| Experimental period | 4-6 weeks (Specific time accord to STR loci /sample number) |
