DNA Sequence Analysis Wild Type Plasmi Site Directed Mutagenesis

Site-directed mutagenesis is an invaluable tool to modify genes and study the structural and functional properties of a protein, based on the structure, function, catalytic mechanism, and catalytic residues of enzymes. Site-directed mutagenesis includes single and combinational mutations. It is usually analyzed by bioinformaticmethods. Single site-directed mutagenesis and multiple mutations have been used to expedite and simplify methods for mutagenes.

What we offer:

Site-Directed Mutagenesis

Site-directed mutagenesis will be carried out on the selected sequence to make specified base changes. The strategy may involve a PCR mutagenesis approach, or an oligonucleotide-directed approach, depending on the needs and specifics of the project.

Screened DNA Sequences

All mutagenesis clones are screened by DNA sequence analysis to ensure that the desired base changes have been made, and to ensure sequence integrity of the remainder of the clone.

Plasmid Preparation

You will receive a mini-scale plasmid preparation, and a stab culture of the strain carrying the plasmid, along with a report documenting restriction analysis and DNA sequence analysis.

Benefit

• Speed—time-to-results is typically less than 3 hours for 3 kb plasmid
• Precision—alter up to 25 nucleotides (nt) when only one site is mutated
• Flexibility—generate substitutions, deletions, or insertions in virtually any plasmid

APPLICATION

1. To study changes in protein activity that occur as a result of the DNA manipulation.
2. To select or screen for mutations (at the DNA, RNA or protein level) that have a desired property
3. To introduce or remove restriction endonuclease sites or tags