Home Products Cellular Molecular BiologyChromatograms Molecular Biology Cdna Library Construction

All Products

Cell Free Protein (8)

Biobasic Gene Synthesis (8)

Oligo Synthesis (8)

Nucleolus Rna Synthesis (9)

Plasmid DNA Preparation (5)

Antibody Discovery (10)

Cellular Molecular Biology (9)

Crispr Cas Gene Editing (10)

Protein Expression And Purification (11)

Molecular Genetic Analysis (9)

Best Products

Gene Synthesis Custom Cell Line Generation

I'm Online Chat Now

Chromatograms Molecular Biology Cdna Library Construction

Large Image : Chromatograms Molecular Biology Cdna Library Construction

Product Details:

Brand Name:	CELLFREE
Model Number:	CF-CM-007

Payment & Shipping Terms:

Packaging Details:	vial
Delivery Time:	4~7 weeks

Contact Now

Detailed Product Description

Starting Material:	Fresh Sample	Starting Information:	Gene Information
Report:	Chromatograms	Deliverables:	Library DNA In Tube Or Plates
Time:	4~7 Weeks	Custom Vector And Protocol:	Aceptable
High Light:	Chromatograms Molecular Biology Cdna Library Construction , Chromatograms Cdna Library Construction

cDNA library construction is a powful tool for gene expression, gene function research, norvel gene characterization, and many other applications. But obtain high-quality cDNA libraries is time-consuming and can be very challenging.

Conventional phage display technique meets obstacles in constructing complex cDNA libraries, such as low concentration of target mRNA, frequent in-frame stop codons due to the 3’ region of poly(A) tail, and constrained structure of original mRNA. These factors result in a poor yield of functional clones, which hinders the development of phage display cDNA libraries for decades.

Standard cDNA Library

Normalized cDNA Library

Subtractive cDNA Library

> 1 x 10⁶ primary clones
Average insert size > 1 kb with > 85% recombinant rate
Size-selected libraries are offered
15 random clones are tested by PCR or enzyme digestion for QC
Library provided in glycerol or DMSO

Normalization is carried out at various Cot levels (Cot₁₀, Cot₅₀ or Cot₅₀₀) according to relative abundance to genes that needs to be normalized
Percent Normalization is tested by:
- Relative PCR for house keeping genes and low abundant genes
- Colony/plaque hybridization of 3,000 ~ 30,000 clones before and after normalization
- DNA sequencing of 96 random cDNA clones
Guarantee to provide > 1x10⁶ primary clones
Average inserts size > 1 kb and over 85% recombinant rate
Libraries can be made directionally in a vector of your choice

Subtraction is carried out according to relative abundance to genes that needs to be subtracted
Percent Subtraction are tested by:
- Relative PCR for house keeping genes and low abundant genes
- Colony/plaque hybridization of 3,000~ 30,000 clones before and after subtraction
- DNA sequencing of 96 random cDNA clones
Guarantee to provide 1x10⁶ primary clones
Average inserts size > 1 kb and over 85% recombinant rate
Libraries can be made directionally in a vector of your choice