Product Details:
Payment & Shipping Terms:
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| Starting With: | Sequencing Information | Deliverables: | 1-2μg DNA Or 5-10μg Plasmid |
|---|---|---|---|
| Report: | Certificate Of Analysis | Sequencing Trace Files: | Available |
| Pooled Ss Oligo Libraries: | 8~15 Working Days | Pooled Ds PCR Libraries: | 20~40 Working Days |
| High Light: | Protein Mutant Libraries Cell Molecular Biology,Gene Mutant Library Cell Molecular Biology,2μg DNA Cell Molecular Biology |
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CELLFREE's high-throughput gene synthesis, mutagenesis and protein production platforms enable us to speed up your project. Our gene mutant library construction service consists of gene synthesis, mutagenesis, sub-cloning, pilot scale protein expression using the E. coli system, and final protein purification.
CELLFREE is able to provide the following mutant library construction services for your scientific research:
Site-directed Mutagenesis Libraries
By combining de novo gene synthesis and site-directed mutagenesis technique, Creative Biogene can offer the most advanced site-directed mutagenesis library construction services. In these libraries, any given residue can be substituted with any of other 19 common amino acids.
Scanning Point Mutation Libraries
Scanning point mutation is a systematic means of improving protein performance. It outperforms standard alanine/cysteine scanning that it is able to substitute each amino acid with all 20 amino acids simultaneously. This technique enables to provide a detailed profile of each amino acid at the position.
Randomized and Degenerated Libraries
With the help of advanced degenerate oligonucleotide techniques, Creative Biogene can construct any form of randomization or degenerate of full-length gene in an interested DNA fragment. The mutation frequency can be set to any value between 1 and 12 mutations per kb.
| Mutant Type | Capacity | Quality Control | Deliverable |
|---|---|---|---|
| Site-directed Mutagenesis Library | Individual, 100% sequence-verified clones | Certificate of Analysis (COA) : 1.Restriction digest map 2.Sequence trace data with alignment 3.Sequence files of the synthetic gene alone or subcloned into a vector |
2-5 µg of lyophilized plasmid DNA |
| Scanning Library | Individual, 100% sequence-verified clones | Certificate of Analysis (COA) : 1.Restriction digest map 2.Sequence trace data with alignment 3.Sequence files of the synthetic gene alone or subcloned into a vector |
2-5 µg of lyophilized plasmid DNA |
| Randomized Library | Ready-to-clone PCR Fragment Library or Cloned Pooled Library Up to 109 variants | Sequence verification of a pre-determined number of clones (with statistical analysis) based on library size | PCR fragment Library: 2-4 µg of linear ds DNA (ready-to-clone via 5’ and 3’ restriction sites if desired) Cloned pool Library: 1. 5-10 µg of lyophilized plasmid DNA 2.Glycerol stock of total library with up to 109 transformants |
| Combinatorial Library | Ready-to-clone PCR Fragment Library or Cloned Pooled Library Up to 109 variants | Sequence verification of a pre-determined number of clones (with statistical analysis) based on library size | PCR fragment Library: 2-4 µg of linear ds DNA (ready-to-clone via 5’ and 3’ restriction sites if desired) Cloned pool Library: 1. 5-10 µg of lyophilized plasmid DNA 2.Glycerol stock of total library with up to 109 transformants |
| Truncation Library | Individual, 100% sequence-verified clones | Certificate of Analysis (COA) : 1.Restriction digest map 2.Sequence trace data with alignment 3.Sequence files of the synthetic gene alone or subcloned into a vector |
2-5 µg of lyophilized plasmid DNA |
