2μg RNA 2 μg DNA High Throughput Fresh Cell NGS Next Generation Sequencing

Introduction

High-throughput sequencing is a cutting-edge tool for quantitative trait gene (QTG) cloning, which is dependent on the mutant gene map of a large-scale segregation population as the clone complex trait.High-throughput sequencing methods generate large amounts of sequence data and require robust computational tools for further analysis. CELLFREE provides algorithms to support common analysis workflows for Next-Generation Sequencing (NGS) data, such as filtering and trimming reads, mapping reads to references, counting the number of reads mapped to genomic features, and performing statistical analyses.

Our services include capture chip preparation, library preparation, sample validation, as well as sequencing and bioinformatics analysis.

Service Type

• Genome Sequencing
• Transcriptome Sequencing
• microRNA Sequencing
• PCR Amplicon Sequencing
• 16S Metagenome Sequencing

Services content

• 2x 300bp read length
• up to 12Gb per flow cell

1. Metagenome analysis via whole genome shotgun sequencing or 16S rDNA sequencing
2. De novo sequencing of all sequences (genome size up to 40 Mb) including sequence assembly
3. Re-sequencing and comparative genomics
4. Transcriptome analysis by generating standard or customised cDNA libraries (e.g. mRNA and micRNA libraries)

• 1.75 bp or 2x150 bp read length
• Up to 120 Gb per flow cell 

1. Genome sequencing to discover SNPs and chromosomal rearrangements
2. Re-sequencing including sequence assembly and alignment to a reference genome
3. Transcriptome analysis, generation of expression profiles, detection of splice variants etc.
4. Targeted re-sequencing of enriched genomic regions of interest (e.g. exomes, chromosomal intervals etc.)
5. Genotyping by Sequencing for all species types (with or without reference genomie)