20 ng/μl MRPP 20 μl Metagenome Sequencing Molecular Genetic Analysis

Introduction

Over 99% of natural microorganisms can not be isolated and cultured clonally. Traditional isolation and culture-dependent methods have limited the study of microorganisms in their natural environment. Metagenomic studies of genetic material that is directly recovered from environmental samples, have benefited greatly from advanced NGS technology as a method for the exploration of microbial biodiversity.

CELLFREE's metagenomics survey service applies whole genome shotgun sequencing of DNA isolated from environmental samples, with the advantages of high throughput and high coverage. It can provide information not only on species composition and abundance, but also on functional genes, gene differences between samples, metabolic pathways and gene resource mining for bioactive products.

Advantage

• Highly experienced: We have completed over 400 metagenomic sequencing projects for our customers, and have published multiple metagenomic studies.
• Outstanding service: We provide high-quality sequencing, an efficient standard workflow, and bioinformatics analyses at a cost-effective price.
• Effective methodology: Our techniques enhance the generation of data from low-abundance species.
• Comprehensive analysis: Expert bioinformatics analysis with three databases (KEGG, eggNOG, CAZy) provides comprehensive data on annotated genes and metabolic pathways.

Data Quality Guarantee

• For each sample, 6Gb and 12Gb packages are offered, with Q30 ≥ 80%.

List of Analyses

• Our standard analysis package includes gene prediction, function annotation, and taxonomic annotation, mPATH, heatmaps, PCA, Krona, cluster analysis, MetaStats, and OG-Taxa.
• Our advanced analysis package includes MRPP, ANOSIM, NMDS (Non-metric Multidimensional Scaling), CCA/RAD, and LEfSe (LDA Effect Size).

Sample Requirements

• DNA amount: ≥ 1 μg
• DNA concentration: ≥ 20 ng/μl
• DNA volume: ≥ 20 μl
• Purity: No degradation, no RNA contamination

Turnaround Time

• Within 25 working days from verification of sample quality (without data analysis)
• Additional 10 working days for data analysis

Recommended Sequencing Depth

• Two strategies: 6 Gb raw data or 12 Gb raw data