Product Details:
Payment & Shipping Terms:
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| Starting Information: | Target Gene Information | Report: | Experiment Report |
|---|---|---|---|
| Small-scale Lentivirus Packaging: | 100 µL | Large-scale Lentivirus Packaging: | 200 µL |
| Deliverable: | Lentivirus Stock | Lentivirus Purification: | Ultracentrifugation.chromatography |
| High Light: | Custom Lentivirus Production Molecular Genetic Analysis,Lentivirus Stock Molecular Genetic Analysis,200 µL Molecular Genetic Analysis |
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Introduction
With the rapid development of the theory and technology of molecular biology (especially the recombinant DNA technology), people can construct various vectors, clone and analyze target genes in the laboratory, so that diseases can be deeply studied at the molecular level. Therefore, gene diagnosis, gene therapy and other technologies are born, so it is particularly critical to select the appropriate and efficient gene delivery medium system, and viral vector has become the hot spot of gene therapy vector research at present. We can provide adenovirus, lentivirus packaging technical services.
CELLFREE offers a lentiviral packaging service for the production of high-quality, high-titer, ready-to-use lentiviral particles. Using a lentivector construct, this service produces VSV-G pseudotyped lentiviral particles at the specified titer level measured as active infectious units (IFU/ml) in just three to four weeks.
You can order lentiviral packaging services using lentivectors provided by you as well as lentivectors constructed by us to obtain ready-to-use particles at various titer levels. We are also happy to customize your order, but please be aware that the prices and delivery times will vary based on the complexity of the order.
Benefits
• Ready-to-transduce lentivirus in three to four weeks
• Ultra-high titer virus made for in vivo and stem cell applications
• Highly concentrated viral preparations
• Precise, active virus titer measurements by qPCR included
• Three different titer levels and flexible volumes available
Customer offer
Target gene information
We provide
The lentiviral packaging experiment report provides 1*108 TU/ml virus (1 ml), control virus (200 μl), and the like.
Service specification
|
Services |
Quantity |
results |
Cycle |
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|
Vector construction |
ShRNA vector of the target gene |
1 |
Report |
1 to 2 weeks |
|
Target gene shRNA vector Subscription |
5 |
Report(4 object +1 negative contrast) |
2 to 3 weeks |
|
|
siRNA lentiviral screening |
Buy cell recovery and training |
1 |
Cell state Image |
2 working days |
|
Slow virus infection experiments |
1 |
The infection efficiency images and analysis |
3 working days |
|
|
shRNA design |
3 |
Target information |
1 working days |
|
|
shRNA vector construction |
4 |
Vector Construction Report |
2 to 3 weeks |
|
|
Lentiviral packaging |
5 |
The virus liquid and the titer test report |
2 to 3 weeks |
|
|
The slower virus infection ( interference filter) |
4 |
Picture |
1 to 2 weeks |
|
|
qRT-PCR primer design and verification |
2 |
Primer sequences ( containing an internal reference ) |
1 weeks |
|
|
qRT-PCR detect |
3*5 |
Experimental data and analysis |
1 to 2 weeks |
|
|
Western Blot detect |
5 |
Experimental data and analysis ( primary antibody ) |
1 to 2 weeks |
|
|
Total price |
/ |
/ |
7 to 10 weeks |
|
|
Establish stable Filter cell lines |
The effective siRNA lentiviral repeated infection ( positive + negative ) |
2 |
Picture |
1 to 2 weeks |
|
Stable cell strain was amplified ( positive + negative ) |
2 |
Cell picture |
3 to 4 weeks |
|
|
qRT-PCR detect |
3*2 |
Experimental data and analysis |
1 to 2 weeks |
|
|
Western Blot detect |
3*2 |
To provide an anti- experimental data analysis (customer ) |
1 to 2 weeks |
|
|
Infected cells (25T flasks , positive ( two kinds ) + negative , each bottle ) |
|
Cells and photo |
1 to 2 weeks |
|
|
Total |
/ |
/ |
6 to 9 weeks |
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